AI Article Synopsis
- Eukaryotes use ATP-dependent chromatin-remodeling complexes to control gene expression, and inositol polyphosphates can influence these complexes' activities.
- Inositol hexakisphosphate (IP6) prevents nucleosome mobilization in certain complexes, like NURF, ISW2, and INO80, while inositol tetrakisphosphate (IP4) and inositol pentakisphosphate (IP5) actually enhance nucleosome mobilization in the SWI/SNF complex.
- Mutations in genes responsible for creating these inositol polyphosphates negatively affect transcription, linking these molecules to chromatin remodeling and gene expression.
Article Abstract
Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin-remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakisphosphate (IP4) and inositol pentakisphosphate (IP5). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP4, IP5, and IP6 impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.
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| http://dx.doi.org/10.1126/science.1078068 | DOI Listing |
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