Effect of sodium orthovanadate on glycosylation of the phosphopeptidomannans involved in the cell-cell aggregation of the yeast Kluyveromyces bulgaricus.

In studies of yeast flocculation it has been found that low concentrations of vanadium contained in sodium orthovanadate do not affect the growth and the cell-cell adhesion of the yeast Kluyveromyces bulgaricus, whereas high concentrations delay the growth of the yeasts and strongly inhibit flocculation. Moreover, higher sensitivity to Hygromycin B and calcofluor white was taken to imply altered cell wall integrity which is supported by compositional analysis of the extracted phosphopeptidomannans. Yeasts grown on sodium orthovanadate show a decrease in the percentage of phosphopeptidomannans and their compositions. It is proposed that the vanadium contained in sodium orthovanadate has a similar conformation to phosphorus and competes with phosphorus in phosphorylated compounds. The decrease of carbohydrate components and phosphorus linking to phosphopeptidomannans detected may alter their structure and modify ligand binding properties.