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Fabrication of macroporous cryogels as potential hepatocyte carriers for bioartificial liver support.

Colloids Surf B Biointerfaces

December 2015

Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur 208016, UP, India. Electronic address:

Two different cryogels composed of copolymer of acrylonitrile (AN) and N-vinyl-2-pyrrolidone (NVP) (poly(AN-co-NVP)) and interpenetrated polymer networks (IPN) of chitosan and poly(N-isopropylacrylamide) (poly(NiPAAm)-chitosan) were fabricated by gelation at sub-zero temperatures. The two cryogels possess an interconnected network of macropores of size 20-100 μm and efficient transport properties as determined by physiochemical analysis. Both cryogels support in vitro growth and function of fibroblasts (COS-7) and human liver hepatocarcinoma cells (HepG2).

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Objective: To construct an off-line hybrid bioartificial liver supporting system with human liver cell line, and study it's effect on the plasma from patients with liver failure.

Methods: We established the bioreactor using Psu-2s (Fresenius) cultured with Hep G2 cell transfected with human augmenter of liver regeneration (hALR) gene, then constructed a hybrid bioartificial liver supporting system, at last using the bioartificial liver support system to purify the plasma treated 2 hours with serum bilirubin absorbent, separated from acute on chronic liver failure patients infected by hepatitis B virus.

Results: Bioreactor was successful constructed.

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Unlabelled: An automatic control device in Bioartificial Liver Support System is designed and implemented. Details of the hardware structure, design of the control system and the applied software in the device are described. Main controller uses the WS-843AW industrial control computer; precision electromagnetic valves are selected as implements to control the flux of three-gases (oxygen, carbon dioxide and nitrogen).

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Objective: To develop an online cell monitoring system for rapid understanding the cell's viability and functional activity, hence appropriate measures could be adopted in time.

Methods: Traditional off line technique was changed into online monitoring in this instrument for rapid detecting albumin and urea. Monocolor light source and integrated photoelectric sensor were adopted in this system.

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