AI Article Synopsis
- Ren-1 and Ren-2 renin are found in the kidneys of all mice and some submandibular glands, and a study explored the effectiveness of modified PAM staining to identify Ren-1 specifically.
- The study involved various mouse strains and techniques, comparing PAM staining with immunohistochemistry to confirm the presence of renin, particularly in juxtaglomerular (JG) cells.
- Results indicated that W-PAM staining was effective in detecting Ren-1 renin in mouse kidneys but was not applicable to Ren-2 or tissues from rats and humans, highlighting W-PAM as a useful method for Ren-1 detection.
Article Abstract
Background: Ren-1 and Ren-2 renin are expressed in the kidneys of all mice and in the submandibular gland of several mouse strains. The present study determined the usefulness of modified periodic acid silver-methenamine (PAM) staining for the specific detection of Ren-1 renin.
Methods: Conventional paraffin sections were prepared from kidneys of ICR, BALB/cA, C57BL/6Cr, C3H/HeN, DBA/2Cr, angiotensin II type 1a receptor gene knockout (AT1aKO) mice, Wistar rats and a human, and submandibular glands of C57BL/6Cr and DBA/2Cr mice. Sections were analyzed for the presence of renin using PAM and immunohistochemistry. PAM reactions were terminated at generally or weakly intense (weak PAM staining; W-PAM). In addition, kidneys of DBA/2Cr mice were fixed using various fixatives (formalin, PFA, PLP, Zamboni's, Bouin's, or Carnoy's) and treated using identical procedures.
Results: Although PAM-positive reactions were observed in juxtaglomerular (JG) cells, W-PAM reactions were particularly specific for these cells. These findings were observed in all mouse strains. Immunohistochemistry using mirror sections suggested that a W-PAM-positive reaction detected renin. This hypothesis was confirmed by the results from AT1aKO mice. Briefly, W-PAM detected an expansion of renin-positive areas in AT1aKO mice. Rat and human kidneys and mouse submandibular glands were negative for W-PAM. Levels of JG cell detection by W-PAM were similar in samples fixed in formalin, PFA, PLP, or Zamboni's.
Conclusions: The present findings show that W-PAM can identify Ren-1 renin, but not Ren-2, rat or human renin. The W-PAM method is useful for the specific detection of Ren-1 renin.
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| http://dx.doi.org/10.1046/j.1523-1755.2002.00688.x | DOI Listing |
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