We have detected granular and filamentous inclusions that are alpha-synuclein- and ubiquitin-immunoreactive in the cytoplasm of dopaminergic and cortical neurons of C57/black mice treated chronically with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and probenecid. The immunoreactive aggregates only become evident several weeks after large-scale dopaminergic cell death and a downregulation of alpha-synuclein gene expression. Numerous lipofuscin granules accumulate alpha-synuclein in the nigral and limbic cortical neurons of treated mice. These data provide evidence that insoluble proteins, such as alpha-synuclein, build up as granular and filamentous inclusions in dopaminergic neurons that survive the initial toxic MPTP insult. They further suggest that defective protein degradation rather than altered gene expression underlies deposition of alpha-synuclein and that abundant lysosomal compartments are present to seal off the potentially toxic material.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0006-8993(02)03514-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Aerobic granules extraction inhibits overgrowth of filamentous bacteria during start-up of aerobic granular sludge.
Bioresour Technol
January 2025
School of Resources and Environment, Hubei Key Laboratory of Regional Development and Environmental Response, Hubei University, Wuhan 430062, China.
In aerobic granular sludge (AGS) system, N-acyl homoserine lactones (AHLs) can effectively regulate the community structure and control filamentous bulking. It would be economically feasible to make mature granules into AHLs-rich AGS extract (AE) to replace synthesized AHLs. In this study, two SBRs were run in a fully aerobic environment and a short cycle (4 h) to culture AGS: R1 with AE adding; R2 served as control.
View Article and Find Full Text PDFDeciphering intricate associations between vigorous development and novel metabolic preferences of partial denitrification/anammox granular consortia within mainstream municipal wastewater.
Bioresour Technol
January 2025
National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, PR China. Electronic address:
There is limited understanding of the granular partial denitrification/anammox (PD/A) microbiota and metabolic hierarchy specific to municipal wastewater treatment, particularly concerning the multi-mechanisms of functional differentiation and granulation tendencies under high-loading shocks. Therefore, this study utilized fragmented mature biofilm as the exclusive inoculum to rapidly establish a granular PD/A system. Following long-term feeding with municipal wastewater, PD/A process reached a total nitrogen removal efficiency of 97.
View Article and Find Full Text PDFLight intensity-regulated glycogen synthesis and pollutant removal in microalgal-bacterial granular sludge for wastewater treatment.
Water Res
March 2025
Engineering Laboratory of Low-Carbon Unconventional Water Resources Utilization and Water Quality Assurance, College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, PR China.
As light intensity plays a pivotal role in the microalgal-bacterial granular sludge (MBGS) process, understanding its impact on system performance and energy dynamics is essential. This study investigated the effects of varying light intensities (20, 100, 200, and 300 μ mol/m²/s) on the performance of MBGS in urban wastewater treatment, with a particular focus on glycogen accumulation and pollutant removal. The results demonstrated that light intensity significantly influenced microbial community structure, glycogen accumulation, and pollutant removal efficiency.
View Article and Find Full Text PDFDirect development of microalgae-bacterial granular sludge system by seeding pre-made microalgae-dewatered sludge granules: Performance and mechanism analysis.
Environ Res
February 2025
School of Civil and Environmental Engineering, Ningbo University, Ningbo, 315211, China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 2W2, Canada.
Microalgae-bacterial granular sludge (MBGS) process has great potential in achieving carbon neutrality and energy neutrality, but rapidly cultivating MBGS remains challenging. To address this challenge, this study proposes a new strategy to develop MBGS systems using pre-made granules from microalgae and dewatered sludge. The results indicate that using pre-made microalgae-dewatered sludge granules (M-DSG) as inoculants can directly develop MBGS system, with M-DSG maintaining a relatively stable granular structure, and ultimately achieving pollutant removal efficiencies of 94.
View Article and Find Full Text PDFMicroscopic investigation of chemical and physical alteration behaviors in sandstone minerals induced by CO-HO-rock interactions during CO saline aquifer storage.
Sci Total Environ
January 2025
School of Petroleum Engineering, China University of Petroleum (East China), Qingdao 266580, PR China. Electronic address:
CO saline aquifer storage represents a promising strategy for mitigating the environmental impact of greenhouse gas emissions. However, the long-term effects of CO dissolved in formation water on rock minerals remain insufficiently understood. This study utilizes cast thin section analysis, scanning electron microscopy, and energy dispersive spectrometry techniques to perform a comprehensive microscopic investigation on this issue.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!