AI Article Synopsis
- The Nps1p/Sth1p protein is crucial for the RSC complex in yeast, helping change nucleosome structure by using ATP energy.
- A temperature-sensitive mutant form of NPS1, called nps1-13, was found to disrupt interactions within the RSC complex and increase cell sensitivity to DNA damage.
- Lower levels of NPS1 expression also led to increased DNA damage sensitivity, highlighting the bromodomain's role in maintaining RSC stability and the need for adequate RSC levels to handle DNA repair.
Article Abstract
The essential Nps1p/Sth1p is a catalytic subunit of the nucleosome-remodeling complex, RSC, of Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defined. We have isolated a temperature-sensitive mutant allele of NPS1, nps1-13, which has amino acid substitutions within the bromodomain. This mutation perturbed the interaction between the RSC components and enhanced the sensitivity of the cells to several DNA-damaging treatments at the permissive temperature. Reduced expression of NPS1 also caused DNA damage sensitivity. These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damage.
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| http://dx.doi.org/10.1016/s0014-5793(02)03504-4 | DOI Listing |
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