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Nitrous oxide production via enzymatic nitroxyl from the nitrifying archaeon .
Proc Natl Acad Sci U S A
January 2025
Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, NY 14853.
Ammonia oxidizing archaea (AOA) are among the most abundant microorganisms on earth and are known to be a major source of nitrous oxide (NO) emissions, although biochemical origins of this NO remain unknown. Enzymological details of AOA nitrogen metabolism are broadly unavailable. We report the recombinant expression, purification, and characterization of a multicopper oxidase, Nmar_1354, from the AOA .
View Article and Find Full Text PDFCoarse bubble mixing in anoxic zone greatly stimulates nitrous oxide emissions from biological nitrogen removal process.
Water Res X
December 2024
School of Energy and Environment, City University of Hong Kong, Hong Kong SAR, China.
The biological nitrogen removal process in wastewater treatment inevitably produces nitrous oxide (NO), a potent greenhouse gas. Coarse bubble mixing is widely employed in wastewater treatment processes to mix anoxic tanks; however, its impacts on NO emissions are rarely reported. This study investigates the effects of coarse bubble mixing on NO emissions in a pilot-scale mainstream nitrite shunt reactor over a 50-day steady-state period.
View Article and Find Full Text PDFInsights into the subdaily variations in methane, nitrous oxide and carbon dioxide fluxes from upland tropical tree stems.
New Phytol
January 2025
Centre of Excellence PLECO (Plants and Ecosystems), Department of Biology, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium.
Recent studies have shown that stem fluxes, although highly variable among trees, can alter the strength of the methane (CH) sink or nitrous oxide (NO) source in some forests, but the patterns and magnitudes of these fluxes remain unclear. This study investigated the drivers of subdaily and seasonal variations in stem and soil CH, NO and carbon dioxide (CO) fluxes. CH, NO and CO fluxes were measured continuously for 19 months in individual stems of two tree species, Eperua falcata (Aubl.
View Article and Find Full Text PDFLess toxic combined microplastics exposure towards attached Chlorella sorokiniana in the presence of sulfamethoxazole while massive microalgal nitrous oxide emission under multiple stresses.
J Hazard Mater
January 2025
MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, PR China.
Microalgae-based wastewater treatment could realize simultaneous nutrients recovery and CO sequestration. However, impacts of environmental microplastics (MPs) and antibiotic co-exposure on microalgal growth, nutrients removal, intracellular nitric oxide (NO) accumulation and subsequent nitrous oxide (NO) emission are unclarified, which could greatly offset the CO sequestration benefit. To reveal the potential impacts of environmental concentrations of MPs and antibiotic co-exposure on microalgal greenhouse gas mitigation, this study investigated the effects of representative MPs (PE, PVC, PA), antibiotic sulfamethoxazole (SMX), and nitrite (NO-N) in various combinations on attached Chlorella sorokiniana growth, nutrients removal, anti-oxidative responses, and NO emission originated from intracellular NO build-up.
View Article and Find Full Text PDFTreatment outcomes in adult patients experiencing dental anxiety: A comparative retrospective study.
Spec Care Dentist
January 2025
Department of Periodontology, Faculty of Dental Medicine, Hadassah Medical Center, Hebrew University of Jerusalem, Jerusalem, Israel.
Aims: To assess and compare the effectiveness of pharmacological and cognitive-behavioral modalities-behavioral methods (BM), nitrous oxide inhalation sedation (NOIS), and premedication with Diazepam-for alleviating anxiety during dental treatment, using completed treatment sessions and adverse outcomes as indicators.
Methods And Results: For 239 patients (515 dental records), 18-53 y/o, who underwent dental treatments under anxiety reduction measures, sessions under NOIS were categorized as "nitrous group" (456 sessions) versus premedication with Diazepam or behavioral strategies (control, 59 sessions). Outcomes were completed treatment sessions considering demographic and clinical parameters, and adverse outcomes during and between sessions.
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