AI Article Synopsis
- The study explores various techniques for delivering active proteins into cells, which is crucial for research and developing treatments.
- Using a luciferase reporter assay, the researchers evaluated the effectiveness of different delivery methods, including cationic lipid complexes and protein chimeras.
- Results show that supramolecular complexes are more effective in cell cultures, but their size could hinder their use in living organisms, while certain peptides can also aid in protein delivery.
Article Abstract
Purpose: The intracellular delivery of functionally active protein represents an important emerging strategy for laboratory investigation and therapeutic applications. Although a number of promising approaches for protein delivery have been developed, thus far there has been no attempt to compare the merits of the various deliver technologies. This issue is addressed in the current study.
Methods: In this study we utilize a sensitive luciferase reporter gene assay to provide unambiguous and quantitative evaluation of several strategies for the intracellular delivery of a biologically active protein comprised of the Gal4 DNA binding domain and the VP16 transactivating domain.
Results: Both a cationic lipid supramolecular complex and a poly meric complex were able to effectively deliver the chimeric transcription factor to cultured cells. In addition, protein chimeras containing the Tat cell penetrating peptide, but not those containing the VP22 peptide, were somewhat effective in delivery.
Conclusions: Both supramolecular protein-carrier complexes and protein chimeras with certain cell penetrating peptides can support intracellular delivery of proteins. In the cell culture setting the supramolecular complexes are more effective, but their large size may present problems for in vivo applications.
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| http://dx.doi.org/10.1023/a:1020346607764 | DOI Listing |
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