AI Article Synopsis
- Locked nucleic acids (LNAs) are modified nucleotides with a unique structure that can enhance gene repair by bridging specific parts of the molecule.
- Researchers found that LNA-modified single-stranded DNA can effectively correct single base mutations in yeast by using them in conjunction with genetic vectors.
- The study indicates that while increasing LNA content decreases correction efficiency, pairing LNA vectors with other modified vectors boosts overall gene repair activity, suggesting LNAs could be valuable in targeting genetic corrections.
Article Abstract
Locked nucleic acids (LNAs) are novel base modifications containing a methylene bridge uniting the 2'-oxygen and the 4'-carbon. In this study, LNA-modified single-stranded molecules directed the repair of single base mutations in a yeast chromosomal gene. Using a genetic assay involving a mutant hygromycin-resistance gene, correction of point and frameshift mutations was facilitated by vectors containing an LNA residue on each terminus. Increasing the number of LNA bases on each terminus reduced the correction frequency progressively. When the LNA vector is used in combination with a phosphorothioate-modified vector (74-mer), however, a high level of gene-repair activity occurs; hence, short LNA-based vectors can augment the activity of other types of targeting vectors. These data suggest that oligonucleotides containing locked nucleic acid residues can be used to direct single nucleotide exchange reactions in vivo.
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| http://dx.doi.org/10.1016/s1074-5521(02)00236-3 | DOI Listing |
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