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Ni-induced selective precipitation of His-tagged recombinant proteins shortens purification time while maintaining high yield.
J Biotechnol
January 2025
Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei-shi, Tokyo 184-8588, Japan; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-8-1 Harumi-cho, Fuchu-shi, Tokyo 183-8538, Japan. Electronic address:
Nickel-NTA affinity chromatography is the current standard method for purifying Histagged recombinant proteins. However, this process involves repetitive tasks, can be time-consuming, and reduces protein yield. Here, we present a simple, fast, and handy method for purifying His-tagged proteins using free Ni²⁺.
View Article and Find Full Text PDFIdentification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides.
J Immunol Methods
December 2024
Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou, China; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoon-Ose, Yangzhou University, Yangzhou, China. Electronic address:
Background: Bovine IgG1 Fc receptor (boFcγRI) is a homologue to human FcγRI (CD64) that has three extracellular Ig-like domains and can bind bovine IgG1 with high affinity. Identification of the linear epitope for Fc-binding on boFcγRI provides new insights for the IgG-Fcγ interaction and FcγR-targeting drugs development.
Methods: The boFcγRI molecules were expressed on cell surface of the boFcγRI -transfected COS-7 cells.
Plant Heat Shock Proteins Are More Effective in Enhancing Recombinant Alcohol Dehydrogenase Activity than Bacterial Ones .
Iran J Biotechnol
July 2024
Department of Biotechnology, Sangmyung University, 20 Hongjimun 2-gil, Jongno-gu, Seoul 03016, Korea.
Background: Recombinant proteins produced in the cell factories are used in biological research, pharmaceutical production, and biochemical and agricultural applications. Molecular chaperones, such as heat shock proteins (Hsps), are co-expressed with recombinant proteins to enhance their yield, stability, and activity. When () is used as a cell factory, Hsps are the frequently used co-expression partners.
View Article and Find Full Text PDFHeterologous expression, enzymatic properties, product analysis and molecular docking of assimilative nitrite reductase (NiR) in Bacillus velezensis GXMZU-B1 derived from mariculture.
Int J Biol Macromol
December 2024
Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China. Electronic address:
High concentrations of nitrite stress aquatic animals, leading to significant fish and shrimp deaths as well as environmental pollution. Reducing nitrite levels in high-density aquaculture is crucial for both aquaculture safety and environmental protection. Nitrite reductase (NiR) can rapidly reduce nitrite in water, offering potential applications in aquaculture and water treatment.
View Article and Find Full Text PDFDifferential structural characteristics, physicochemical properties, and calcium-binding capabilities of annexin A2 wild-type versus E53A, E96A, D162A, E247A and D322A mutants.
Arch Biochem Biophys
December 2024
Medical Proteomics Unit, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand. Electronic address:
Annexin A2 (ANXA2) is a Ca-dependent multifunctional protein containing five Ca-binding domains, but their functional significance and difference remain unclear. Herein, glutamic acid (E) or aspartic acid (D) in five Ca-binding domains of canine ANXA2 (98.82 % and 96.
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