AI Article Synopsis
- Triple quadrupole mass spectrometers are crucial for high-throughput quantitation in pharmaceuticals due to their sensitivity and specificity, especially when operated in multiple reaction monitoring (MRM) mode.
- Recent advancements have improved these instruments to achieve mass resolution of less than 0.1 Dalton, addressing past limitations with interference from matrix components or metabolites.
- The paper highlights the benefits of enhanced resolution, particularly in reducing interference during analyses (like with mometasone and PPG), maintaining sensitivity, and demonstrating stability during extended operation.
Article Abstract
Triple quadrupole mass spectrometers, when operated in multiple reaction monitoring (MRM) mode, offer a unique combination of sensitivity, specificity, and dynamic range. Consequently, the triple quadrupole is the workhorse for high-throughput quantitation within the pharmaceutical industry. However, in the past, the unit mass resolution of quadrupole instruments has been a limitation when interference from matrix or metabolites cannot be eliminated. With recent advances in instrument design, triple quadrupole instruments now afford mass resolution of less than 0.1 Dalton (Da) full width at half maximum (FWHM). This paper describes the evaluation of an enhanced resolution triple quadrupole mass spectrometer for high-throughput bioanalysis with emphasis on comparison of selectivity, sensitivity, dynamic range, precision, accuracy, and stability under both unit mass (1 Da FWHM) and enhanced (
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http://dx.doi.org/10.1002/rcm.824 DOI Listing Publication Analysis
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