Objective: To investigate adriamycin (Adr) uptake and distribution features in multidrug-resistant LoVo/Adr cells and explore the drug-resistance mechanism of the cells.
Methods: Adr uptake in LoVo/Adr cells was observed by flow cytometry and the distribution of the drug examined by fluorescence microscope. Immunohistochemical method was employed to detect the expression of P-glycoprotein (P-gp) by the cells.
Results: In comparison with that in LoVo/Adr cells, Adr level in LoVo cells was significantly higher, but after treatment with verapamil, the former cells showed an increased Adr level. Adr distributed mainly in the nucleus in the drug-sensitive LoVo cells, with only a small quantity in the cytoplasm, while in multidrug-resistant LoVo/Adr cells, significantly reduction of Adr quantity in the nucleus and relative increase in the cytoplasm were observed. In response to verapamil treatment, the Adr uptake in LoVo/Adr cells increased and the distribution of the drug was similar to that in sensitive cells. P-gp expression was positive in LoVo/Adr cells, while negative in LoVo cells.
Conclusion: Abnormal Adr uptake and distribution in drug-resistant cells is related to P-gp expression which is one of the mechanisms for multidrug resistance.
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