Objective: To study the projections between the medial region of the nucleus retrofacialis (mNRF) and other respiratory regions in rat brainstem.
Methods: Twenty-five adult SD rats were used in this study, which received anesthesia with sodium pentobarbitone. Retrograde/anterograde tracing methods were employed by unilateral microinjection into mNRF with 30% horseradish peroxidase (HRP, 0.5-1.0 microliter1) in 13 rats. Another 5 rats with injections of HRP and 2 with WGA-HRP into the regions 2 mm off mNRF served as control groups. and with 5% wheat germ agglutinin-conjugated HRP (WGA-HRP, 20-60 nl) in 5 rats (with another 2 receiving the injections as control).
Results: It was found that HRP-labeled neuronal somas and WGA-HRP labeled terminal fibers were found in many respiration-related regions in rat brainstem such as the nucleus tractus solitarius, nucleus ambiguous, gigantocellular reticular nucleus and so forth.
Conclusions: There are comprehensive projections between mNRF and other respiratory regions in rat brainstem, and these respiratory regions may be involved in basic respiratory rhythm regulation via these connections with mNRF.
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