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Hydrodynamic characterization of the FtsZ protein from Escherichia coli demonstrates the presence of linear and lateral trimers.
Anal Biochem
April 2025
Laboratorio de Biología Estructural y Molecular BEM, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Ñuñoa, Santiago, 7800003, Chile; Laboratorio de Biotecnología Vegetal y Ambiental Aplicada, Universidad Tecnológica Metropolitana, Santiago, Chile.
FtsZ is a bacterial protein that plays a crucial role in cytokinesis by forming the Z-ring. This ring acts as a scaffold to recruit other division proteins and guide the synthesis of septal peptidoglycan, which leads to cell constriction. In its native state, the FtsZ protein from Escherichia coli (EcFtsZ) is a multi-oligomer comprising dimers, trimers, tetramers, and hexamers in a dynamic self-association equilibrium depending on its concentration.
View Article and Find Full Text PDFEvaluation of objective methods for analyzing ipsilateral motor evoked potentials in stroke survivors with chronic upper extremity motor impairment.
J Neural Eng
January 2025
Department of Physical Medicine and Rehabilitation, MetroHealth Medical Center, 4229 Pearl Road, Suite N4-13, Cleveland, Ohio, 44109-1998, UNITED STATES.
Ipsilateral motor evoked potentials (iMEPs) are believed to represent cortically evoked excitability of uncrossed brainstem-mediated pathways. In the event of extensive injury to (crossed) corticospinal pathways, which can occur following a stroke, uncrossed ipsilateral pathways may serve as an alternate resource to support the recovery of the paretic limb. However, iMEPs, even in neurally intact people, can be small, infrequent, and noisy, so discerning them in stroke survivors is very challenging.
View Article and Find Full Text PDFPhoto-Cross-linked DNA Structures Greatly Improves Their Serum Nuclease Resistances and Gene Knock-In Efficiencies.
Small Methods
December 2024
Hefei National Research Center for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, 230026, China.
The stabilization and structural integrity of DNA architectures remain significant challenges in their biomedical applications, particularly when inserting functional units into the genome using long single-stranded DNA (lssDNA). To address these challenges, a site-specific photo-cross-linking method is employed. Single-stranded oligonucleotides, containing one or two photosensitive cyanovinylcarbazole nucleoside (K) molecules, are precisely incorporated and cross-linked at the specific sites of ssDNA through base-pairing, followed by rapid UV irradiation at 365 nm.
View Article and Find Full Text PDFImprovement by imagining actions: Bimanual transfer effects after action imagery practice in a sequential reaction time task.
Acta Psychol (Amst)
February 2025
UMIT Tirol - Private University for Health Sciences and Health Technology, Hall in Tyrol, Austria.
Action-imagery-practice refers to the repetitive use of action imagery to improve subsequent performance leading to partially different representation types than action-execution-practice (AEP). This study explored the representation types in kinesthetic action-imagery-practice (K-AIP) and visual action-imagery-practice (V-AIP) in a serial reaction time task using the crossed hand transfer paradigm. 169 participants (age M ± SD = 25.
View Article and Find Full Text PDFMultiscale Elasticity of Epoxy Networks by Rheology and Brillouin Light Spectroscopy.
J Phys Chem B
December 2024
Institute of Electronic Structure and Laser, FORTH, Heraklion 70013, Greece.
The response of soft materials to an imposed oscillatory stress is typically frequency dependent, with the most utilized frequency range falling in the range of 10-10 rad/s. In contrast to most conventional contact techniques for measuring material elasticity, like tensile or shear rheology and atomic force microscopy, or invasive techniques using probes, such as microrheology, Brillouin light spectroscopy (BLS) offers an optical, noncontact, label-free, submicron resolution and three-dimensional (3D) mapping approach to access the mechanical moduli at GHz frequencies. Currently, the correlation between the experimental viscoelastic (at lower frequencies) and elastic (at higher frequencies) moduli has fundamental and practical relevance, but remains unclear.
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