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AI-Enhanced Understanding of Retention Interactions in Supercritical Fluid Chromatography: Neural Network Insights into Retention on Selected Non-Polar Stationary Phases.
Anal Chem
January 2025
Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, 500 05 Hradec Králové, Czechia.
The retention behavior in supercritical fluid chromatography (SFC) remains a complex and poorly understood phenomenon despite the development of various models to explain retention mechanisms. This study aims to deepen the understanding of retention by investigating three distinct stationary phases: high-strength silica octadecyl (HSS C18 SB), charged surface hybrid pentafluorophenyl (CSH PFP), and porous graphitic carbon (PGC) as a nonsilica-based phase. Three mobile phase compositions, i.
View Article and Find Full Text PDFSupercritical fluid extrusion of pea flour and pea protein concentrate: Effects on off-flavor removal and sensory improvement.
J Food Sci
January 2025
Department of Food Science, Cornell University, Ithaca, New York, USA.
This study was intended to provide a novel process that fills a knowledge gap in relation to the enhancement of pulses utilization. The primary goal was to develop an experimental framework for using a high-pressure supercritical fluid extruder (SCFX) as a continuous bioreactor to produce off-flavor reduced and functionally superior pulse flours and protein concentrates in a single step. The current study focused on using SCFX processing to remove off-flavor from pulse flour and protein concentrates, enhancing the quality, acceptability, and marketability of pulse-based products.
View Article and Find Full Text PDFEnergized dispersive guided extraction (EDGE): a promising tool for analytical chemistry applications.
Anal Methods
January 2025
Rede Nordeste de Biotecnologia, Universidade Federal de Sergipe, São Cristóvão, SE 49100-000, Brazil.
Analytical chemistry demands precise sample preparation methods to ensure accurate qualitative and quantitative determinations, especially those capable of clean-up and preconcentration of target analytes. Extraction plays a crucial role in enhancing the selectivity and sensitivity of analytical procedures. Thus, Energized Dispersive Guided Extraction (EDGE) has emerged as an innovative alternative to traditional methods, such as Soxhlet, maceration, and percolation, as well as modern techniques like Accelerated Solvent Extraction (ASE), Supercritical Fluid Extraction (SFE), and Microwave or Ultrasound Assisted Extraction (MAE and UAE).
View Article and Find Full Text PDFLipidomic characterization of polar and non-polar lipids in egg yolk and their anti-inflammatory and analgesic activities in zebrafish.
Food Chem
January 2025
Institute of Traditional Chinese Medicine and Natural Products, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, College of Pharmacy, Jinan University, Guangzhou 510632, People's Republic of China. Electronic address:
In this study, a technique that combines supercritical fluid extraction with ethanol extraction was employed to effectively fractionate egg yolk extracts into non-polar and polar lipids. By utilizing feature based molecular networking (FBMN) and MS-DIAL database search, 35 glycerolipids were identified in the supercritical extract (SE), and 70 glycerophospholipids in the ethanol extract (EE). Following the identification of lipids, a heatmap was generated using R software to visualize the abundance of lipids across both fractions.
View Article and Find Full Text PDFLutein and Zeaxanthin: Source, Extraction, Stability, Bioactivity, and Functional Food Applications.
Curr Pharm Biotechnol
January 2025
Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Saudi Arabia.
Nature has been acknowledged as a fundamental source of diverse bioactive molecules. Among natural carotenoids, lutein, zeaxanthin, and their oxidative metabolites are specifically deposited in the macular region of living organisms. Lutein and zeaxanthin are carotenoids primarily found in green leafy vegetables, eggs, and various fruits.
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