AI Article Synopsis
- Insensitive acetylcholinesterase (AChE) was found to be a resistance mechanism in cotton bollworms that are resistant to monocrotophos.
- Researchers cloned the AChE gene and found it has a signal peptide, a mature protein sequence, and significant similarities to AChE from other insect species.
- Analysis of AChE gene sequences revealed nine mutations, with the Ala/Thr mutation being a key factor in the bollworms' insensitivity to monocrotophos.
Article Abstract
Insensitive acetylcholinesterase was identified as a resistance mechanism by comparing biochemical analysis with a laboratory selected monocrotophos resistant cotton bollworm (RR: 200) and the susceptible strain. The cDNA encoding AChE was cloned by the method of RACE (rapid amplification of cDNA ends). The complete AChE gene deduced from the cDNA consisted of a putative signal peptide of 32 amino acid residues, a mature protein of 615 residues, 5' untranslated regions (UTR) of 315 bp and 3' UTR of 324 bp. The coding sequence had a high degree of homology to the AChE from other insect species reported in the GenBank. After comparing analysis of the entire AChE gene sequence from 5 resistant and 6 susceptible cotton bollworm individuals, nine mutations were identified. One of them, the Ala/Thr mutation, is likely to be responsible for the AChE insensitivity to monocrotophos.
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