In plant, evocation of secondary metabolism is associated with complex biochemical and molecular events that are regulated by developmental and environmental factors. In order to get more information about Taxol biosynthesis, comparison of mRNA populations from Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase were performed by mRNA differential display. The results suggested that genes specifically expressed in the Taxol-synthesis phase might be involved in Taxol biosynthesis.
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Enhanced taxane production and secretion from Taxus baccata cell culture by adding dimethylsulfoxide.
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Department of Secondary Metabolite, Agricultural Biotechnology Research Institute, Central Region of Iran, Isfahan, Iran.
The ability of an aprotic solvent, dimethylsulfoxide (DMSO), to induce taxane synthesis and release from cell suspension culture of Taxus baccata was examined. The results showed that applying DMSO in optimal conditions not only led to enhancement in taxane excretion from the cells but also led to improvement in taxol synthesis. Maximum yields for taxol [3.
View Article and Find Full Text PDFIdentification of a cDNA clone specific for the taxol synthesis phase of Taxus chinensis cells by mRNA differential display.
Nat Prod Res
August 2004
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
In order to identify genes related to Taxol biosynthesis, the mRNA differential display method was employed to compare mRNA populations from suspension cultured Taxus chinensis cells before and after beginning to produce Taxol. From a total display of about 4000 PCR products, 104 were derived from cells during the Taxol synthesis phase but not the non-Taxol synthesis phase. These products were cloned, and one such cDNA clone, named TS1, was confirmed to be specifically expressed in the Taxol synthesis phase by northern blot analysis.
View Article and Find Full Text PDF[Differences in gene expression between Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase].
Sheng Wu Gong Cheng Xue Bao
July 2002
School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
In plant, evocation of secondary metabolism is associated with complex biochemical and molecular events that are regulated by developmental and environmental factors. In order to get more information about Taxol biosynthesis, comparison of mRNA populations from Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase were performed by mRNA differential display. The results suggested that genes specifically expressed in the Taxol-synthesis phase might be involved in Taxol biosynthesis.
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