Neurological damage is a serious problem after cardiac arrest and resuscitation. We used a rat cardiac arrest model to test the ability of a post-ischemic infusion of adenosine triphosphate-magnesium chloride (ATP-MgCl(2)) to sustain cortical protein synthesis after 7 min global ischemia. We used norepinephrine (NE) to block the vasodilatory action of ATP, and a trace of vanadate to simulate the equine-derived ATP Chaudry used to protect against ischemia or hemorrhage in other organs. Our ATP 'cocktail' (3 ml of 4 mg/ml ATP-MgCl(2), 18 microg/ml NE, 2.4 microg/ml vanadate, infused intravenously over 18 min) tripled post-ischemic protein synthesis. ATP without vanadate, and vanadate without ATP, both had lesser but still significant effects. This ATP 'cocktail' may be useful as a neuroprotectant after cardiac arrest and resuscitation.
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