Objective: To prepare a tissue-engineered scaffold material using collagen as the matrices and to study the blood compatibility and tissue biocompatibility of this material.
Methods: Physical, chemical and physical/chemical methods were used for the crosslinking of the collagen.
Results: Dynamic blood clotting tests indicated that the blood clotting index (BCI) of the crosslinked collagen materials prepared by different means decreased as their contact with the blood was prolonged, and the collagen material obtained after crosslink through 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide method showed the highest BCI after contact with the blood within certain length of time. Hemolysis ratios of all the crosslinked collagen materials were shown to be much lower than 5%, well conforming to the requirement of biomaterials. Scanning electron microscopy showed that the platelets attached to the surface of the crosslinked collagen materials, having a fairly small number, were not morphologically distorted.
Conclusion: The collagen materials obtained by the 3 crosslink methods have good blood compatibility. The cells grow well on the surfaces of the materials, indicating their good biocompatibility.
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