Turnaround time for Papanicolaou (Pap) tests became an important service quality issue at our institution. We studied Pap test turnaround time using engineering process improvement tools and benchmarked turnaround time against data published as a College of American Pathologists Q-Probes study. An IDEF3 process map revealed the complexity of the Pap test process and the opportunities for process improvement. We used these data and the action-research method to initiate changes in cytopathology laboratory operations with the goal of reducing turnaround time. Before intervention, mean Pap test turnaround time was highly variable; during a 6-month period, monthly means ranged from 2.5 to 10.8 days. A cycle time study conducted over a 2-week period validated these data. After system improvements were implemented, the monthly mean turnaround time decreased and became more consistent, with 11 of 12 months having a mean turnaround time of 3 days or less (range, 1.5-3.9 days). Our study illustrates the value of publishing Q-Probes data for use as external benchmarks and the benefits of using tools from other disciplines to improve laboratory processes.
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http://dx.doi.org/10.1309/20NX-VJUK-VADH-51T8 | DOI Listing |
Diagnostics (Basel)
March 2025
Armauer Hansen Research Institute (AHRI), Addis Ababa P.O. Box 1005, Ethiopia.
Bacteriological culture has been a widely used method for the detection of meningococcus, but it has low sensitivity and a long turnaround time. Molecular detection targeting capsule transport A ( gene has been used, but over 16% of meningococcal carriage isolates lack , resulting in false-negative reports. The Cu-Zn superoxide dismutase gene () is specific to , and is not found in other , making it a useful target for improved detection of non-groupable meningococci without intact .
View Article and Find Full Text PDFDiagnostics (Basel)
March 2025
Parasitology Reference and Research Laboratory, National Microbiology Centre, Instituto de Salud Carlos III, 28220 Majadahonda, Spain.
: Many tropical diseases such as malaria, Chagas, human African Trypanosomiasis, and Lymphatic filariasis coexist in endemic countries, affecting more than 1 billion people worldwide, and are recognised as major global vector-borne diseases. Tackling this disease requires an accurate diagnosis that is sensitive, specific, and rapid. This study aimed to describe and validate a new highly sensitive and specific multiple-analysis system that can effectively detect numerous etiological agents in a single test.
View Article and Find Full Text PDFCancers (Basel)
February 2025
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China.
Background/objectives: Mesenchymal-epithelial transition () gene amplification is a critical biomarker in non-small cell lung cancer (NSCLC), significantly influencing treatment decisions and prognostic evaluations. However, current detection methods such as fluorescence in situ hybridization (FISH) and next-generation sequencing (NGS) have limitations in speed, cost, and specificity, particularly when distinguishing between focal amplification and polysomy.
Methods: This study introduces a novel digital PCR (dPCR) assay designed not only to detect amplification but also to differentiate between its focal and non-focal subtypes.
Phytopathology
March 2025
Michigan State University, Dept. Plant, Soil and Microbial Sciences, 105 CIPS, East Lansing, Michigan, United States, 48910;
Oak wilt, caused by the fungal pathogen , spreads via root grafts and insect vectors, threating oaks ( spp.) and chestnuts ( spp.) in the United States.
View Article and Find Full Text PDFJ Opioid Manag
March 2025
Department of Emergency Medicine, Baylor Scott and White All Saints Medical Center, Fort Worth, Texas.
Objective: To investigate the potential impact of implementing alternatives to opioids (ALTOs) protocol in a community emergency department (ED) in North Texas. We hypothesize that the ALTO protocol is associated with decreased opioid utilization without affecting patient satisfaction to pain control and ED flow.
Design: A retrospective, single-center, cohort study.
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