AI Article Synopsis
- GroEL protein and groEL mRNA levels were found to be higher in gyrB mutants of Borrelia burgdorferi compared to both wild-type strains and heat-shocked cultures.
- The circular DNA structure in gyrB mutants was more relaxed than that of the wild-type, indicating a difference in DNA topology.
- This study provides the first indirect evidence suggesting that the topology of linear DNA can influence gene expression in bacteria.
Article Abstract
GroEL protein and groEL mRNA transcript were up-regulated in gyrB mutants of Borrelia burgdorferi, a causative agent of Lyme disease. Furthermore, the protein and transcript levels in gyrB mutants were greater than those in experimentally heat-shocked cultures of wild-type B. burgdorferi. Circular DNA in the gyrB mutants was more relaxed than in wild-type cells, although groEL is on the linear chromosome of B. burgdorferi. To our knowledge, this is the first evidence, albeit indirect, for the effect of DNA topology on gene expression from a linear DNA molecule in a bacterium.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC135381 | PMC |
| http://dx.doi.org/10.1128/JB.183.21.6069-6072.2002 | DOI Listing |
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