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Unveiling endothelial cell border heterogeneity: VE-cadherin adherens junction stratification by deep convolutional neural networks.
PLoS One
January 2025
Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands.
Background: Systemic diseases are often associated with endothelial cell (EC) dysfunction. A key function of ECs is to maintain the barrier between the blood and the interstitial space. The integrity of the endothelial cell barrier is maintained by VE-Cadherin homophilic interactions between adjacent cells.
View Article and Find Full Text PDFGenetic dissection of a major locus SC9.1 conferring seed color in broomcorn millet (Panicum miliaceum).
Theor Appl Genet
January 2025
Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, China.
A major locus SC9.1 was identified and finely mapped into a 92.68 Kb region, and longmi004412 was identified as the casual gene regulating brown seed color in broomcorn millet.
View Article and Find Full Text PDFIntroductory Analysis and Validation of CUT&RUN Sequencing Data.
J Vis Exp
December 2024
Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center; Department of Gynecologic Oncology and Reproductive Medicine, University of Texas MD Anderson Cancer Center;
The CUT&RUN technique facilitates detection of protein-DNA interactions across the genome. Typical applications of CUT&RUN include profiling changes in histone tail modifications or mapping transcription factor chromatin occupancy. Widespread adoption of CUT&RUN is driven, in part, by technical advantages over conventional ChIP-seq that include lower cell input requirements, lower sequencing depth requirements, and increased sensitivity with reduced background signal due to a lack of cross-linking agents that otherwise mask antibody epitopes.
View Article and Find Full Text PDFGas-Phase Fluorescence Excitation Experiments on Cryogenically Cold Rhodamine B Cations Linked to Various Amino Acid Esters.
J Am Soc Mass Spectrom
January 2025
Department of Physics and Astronomy, Aarhus University, Aarhus 8000, Denmark.
Förster resonance energy transfer (FRET) is becoming a valuable technique in gas-phase structural biology for identifying local structural motifs and conformations of biological molecules, such as peptides and proteins. This method involves labeling the biomolecule with two dyes, a donor dye and an acceptor dye, that are commonly charged rhodamines. Here we examine how different amino acid (AA) methyl esters linked to the dye via amide linkages can influence the dye transition energy and, consequently, the energy-transfer efficiency, using cryogenic ion fluorescence spectroscopy.
View Article and Find Full Text PDFIdentification of cellular signatures associated with chinese hamster ovary cell adaptation for secretion of antibodies.
Comput Struct Biotechnol J
December 2024
Cell Culture and Fermentation Sciences, BioPharmaceutical Development, AstraZeneca, Cambridge UK.
The secretory capacity of Chinese hamster ovary (CHO) cells remains a fundamental bottleneck in the manufacturing of protein-based therapeutics. Unconventional biological drugs with complex structures and processing requirements are particularly problematic. Although engineered vector DNA elements can achieve rapid and high-level therapeutic protein production, a high metabolic and protein folding burden is imposed on the host cell.
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