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A simple strategy for culturing morphologically-conserved rat hypothalamic tanycytes.
Cell Tissue Res
August 2017
Laboratory of Neurophysiology of the Multidisciplinary Institute of Cell Biology [IMBICE, dependent of the Argentine Research Council (CONICET) and Scientific Research Commission, Province of Buenos Aires (CIC-PBA)], Calle 526 entre 10 y 11, PO Box 403, La Plata, 1900, Buenos Aires, Argentina.
Hypothalamic tanycytes are specialized bipolar ependymal cells that line the floor of the third ventricle. Given their strategic location, tanycytes are believed to play several key functions including being a selective barrier and controlling the amount of hypothalamic-derived factors reaching the anterior pituitary. The in vitro culture of these cells has proved to be difficult.
View Article and Find Full Text PDFBotulinum neurotoxin type A-cleaved SNAP25 is confined to primary motor neurons and localized on the plasma membrane following intramuscular toxin injection.
Neuroscience
June 2017
Department of Biological Sciences, Allergan plc, Irvine, CA 92612, United States. Electronic address:
The mechanism of action of botulinum neurotoxin type A (BoNT/A) is well characterized, but some published evidence suggests the potential for neuronal retrograde transport and cell-to-cell transfer (transcytosis) under certain experimental conditions. The present study evaluated the potential for these processes using a highly selective antibody for the BoNT/A-cleaved substrate (SNAP25) combined with 3-dimensional imaging. SNAP25 was characterized in a rat motor neuron (MN) pathway following toxin intramuscular injections at various doses to determine whether SNAP25 is confined to MNs or also found in neighboring cells or nerve fibers within spinal cord (SC).
View Article and Find Full Text PDFPlacental exosomes and pre-eclampsia: Maternal circulating levels in normal pregnancies and, early and late onset pre-eclamptic pregnancies.
Placenta
October 2016
Nelson R Mandela School of Medicine, School of Laboratory Medicine & Medical Sciences, University of KwaZulu-Natal, Durban, South Africa. Electronic address:
Introduction And Aim: Exosomes are a subtype of extracellular vesicle (20-130 nm) released by biological cells under normal and pathological conditions. Although there have been reports of circulating exosomes in normal pregnancy, the relevance of placental-derived exosomes in normal and abnormal pregnancies still needs to be elucidated. The aim of this study was to quantify total and placental-derived exosomes in maternal plasma from normal (N), early onset- and late onset-preeclampsia (PE).
View Article and Find Full Text PDFPredictors of Abnormal Glucose Tolerance in the Early Postpartum Period in Patients with Gestational Diabetes.
Kurume Med J
April 2017
Department of Obstetrics and Gynecology, Kurume University School of Medicine.
This study was designed to investigate the clinical predictors of abnormal glucose tolerance 5-7 weeks after delivery. Subjects were 155 women diagnosed with gestational diabetes mellitus (GDM) between October 2005 and September 2013 whose pregnancy and delivery were managed at our center. Subjects were divided into a normal glucose tolerance group (NGT; n = 113), or abnormal glucose tolerance group (AGT; n = 42) with borderline or overt diabetes mellitus, based on 75-g oral glucose tolerance test (75 gOGTT) results 5-7 weeks after delivery.
View Article and Find Full Text PDFIdentification of sheep red blood cell (SRBC) surface immune-responsive peptides detected by antisera from SRBC-immunized rats.
J Toxicol Sci
February 2016
Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute, Japan.
To identify the sheep red blood cell (SRBC) surface immune-responsive peptides, immuno-reactive fraction of SRBC was detected by SDS-PAGE and western blot analysis with antisera from SRBC-immunized rats. Then the most intense immuno-reactive band on SDS-PAGE was subjected to nanoLC-ESI-MS/MS analysis, and 17 proteins were identified including membrane proteins of erythrocytes such as band 3 anion transport protein isoform 1 (Anion exchange protein 1; AE-1, CD233), Ammonium transporter Rh type A (Rh type A glycoprotein, CD241) and Ankyrin-1 (ANK-1), Spectrin beta chain. Among them, plasma protein AE-1 (CD233) and Rh type A glycoprotein (CD241) have transmembrane domain and correspond to extracellular region in their sequences.
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