A new type II restriction endonuclease designated PfoI has been partially purified from Pseudomonas fluorescens biovar 126. PfoI recognises the interrupted hexanucleotide palindromic sequence 5'-T downward arrow CCNGGA-3' and cleaves DNA to produce protruding pentanucleotide 5'-ends.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC140558PMC
http://dx.doi.org/10.1093/nar/gnf097DOI Listing

Publication Analysis

Top Keywords

type restriction
8
restriction endonuclease
8
sequence 5'-t
8
5'-t downward
8
downward arrow
8
arrow ccngga-3'
8
pfoi unique
4
unique type
4
endonuclease recognises
4
recognises sequence
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!