Monocyte-derived dendritic cells (DCs) from adult T cell leukemia are impaired in taking up exogenous antigens. To overcome this impairment, we fused unpulsed DCs to human T-lymphotropic virus type I (HTLV-I)-infected CD4(+) T cells (fusion DC-T cells). The efficiency of fusion was 50% and the fusion cells expressed higher HLA-ABC and CD86 Ags than HTLV-I-infected DCs. The fusion DC-T cells stimulated autologous CD4(+) and CD8(+) T cells, but DCs fused to itself or PHA-blasts did not stimulate any subsets. The functionally highest fusion DC-T cells was obtained when a DC and HTLV-I-infected T cells were fused at a ratio of 3:1. Expression of HTLV-Igag Ag on CD4(+) T cells was up-regulated when infected in the presence of 8-azaguanine, and these fusion DC-T cells were quite efficient in induction of higher CD8(+) T cell response. The results suggest that fusion DC-T cells produce functionally competent Ag-presenting cells and may be a likely candidate for immunotherapeutic use.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/viro.2002.1546 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
CTLA4-Ig immunosuppressive activity at the level of dendritic cell/T cell crosstalk.
Int Immunopharmacol
March 2013
Children's Cancer Research Institute, Division of Transplantation Immunology, Vienna, Austria.
Article Synopsis
- CTLA4-Ig is a fusion protein that inhibits T cell responses by blocking the CD28:CD80/86 costimulatory pathway and influences dendritic cell (DC) function.
- The study investigated the effects of CTLA4-Ig on C57BL/6-derived DCs and their ability to stimulate allogeneic Balb/c T cells.
- Findings show that CTLA4-Ig does not impair the stimulatory capacity of DCs when removed prior to T cell interaction, indicating that its immunosuppressive effects are only active during the co-culture with T cells.
Regulation and function of the E-cadherin/catenin complex in cells of the monocyte-macrophage lineage and DCs.
Blood
February 2012
Myeloid Cell Immunology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium.
E-cadherin is best characterized as adherens junction protein, which through homotypic interactions contributes to the maintenance of the epithelial barrier function. In epithelial cells, the cytoplasmic tail of E-cadherin forms a dynamic complex with catenins and regulates several intracellular signal transduction pathways, including Wnt/β-catenin, PI3K/Akt, Rho GTPase, and NF-κB signaling. Recent progress uncovered a novel and critical role for this adhesion molecule in mononuclear phagocyte functions.
View Article and Find Full Text PDFA fusion protein of flagellin and ovalbumin suppresses the TH2 response and prevents murine intestinal allergy.
J Allergy Clin Immunol
December 2011
Division of Allergology, Paul-Ehrlich-Institut, Langen, Germany.
Background: The Toll-like receptor (TLR) 5 agonist flagellin is associated with immunomodulatory functions.
Objective: We sought to investigate whether Listeria monocytogenes-derived flagellin A (flaA) can modulate ovalbumin (OVA)-specific T-cell responses and prevent OVA-induced intestinal allergy.
Methods: Bone marrow-derived myeloid dendritic cells from BALB/c, C57BL/6, or TLR signaling-deficient (MyD88(-/-)) mice were stimulated with rOVA, rflaA, rflaA plus rOVA, or a recombinant fusion protein consisting of rflaA and rOVA (rflaA:OVA).
Attachment and fusion inhibitors potently prevent dendritic cell-driven HIV infection.
J Acquir Immune Defic Syndr
March 2011
Center for Biomedical Research, Population Council, New York, NY 10065, USA.
Dendritic cells (DCs) efficiently transfer captured (trans) or de novo-produced (cis) virus to CD4 T cells. Using monocyte-derived DCs, we evaluated entry inhibitors targeting HIV envelope (BMS-C, T-1249) or CCR5 (CMPD167) for their potency to prevent DC infection, DC-driven infection in T cells in trans and cis, and direct infection of DC-T-cell mixtures. Immature DC-T-cell cultures with distinct mechanisms of viral transfer yielded similar levels of infection and produced more proviral DNA compared with matched mature DC-T-cell cultures or infected immature DCs.
View Article and Find Full Text PDFA therapeutic OX40 agonist dynamically alters dendritic, endothelial, and T cell subsets within the established tumor microenvironment.
Cancer Res
November 2010
Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
Little preclinical modeling currently exists to support the use of OX40 agonists as therapeutic agents in the setting of advanced cancers, as well as the mechanisms through which therapeutic efficacy is achieved. We show that treatment of mice bearing well-established day 17 sarcomas with a novel OX40 ligand-Fc fusion protein (OX40L-Fc) resulted in tumor regression or dormancy in the majority of treated animals. Unexpectedly, dendritic cells (DC) in the progressive tumor microenvironment (TME) acquire OX40 expression and bind fluorescently labeled OX40L-Fc.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!