Photoaccumulation of the PsaB phyllosemiquinone in photosystem I of Chlamydomonas reinhardtii.

Biochim Biophys Acta

School of Biological Sciences, Queen Mary, University of London, Mile End Road, E1 4NS, London, UK.

Published: October 2002

Photoaccumulation of membrane preparations of Chlamydomonas reinhardtii at pH 8 and 220 K reduces the primary and secondary electron acceptors in the Photosystem I (PSI) reaction centre, and produces a maximum of two spins per P700(z.rad;+). Proton electron nuclear double resonance (ENDOR) spectra demonstrate that the phyllosemiquinone produced is that attributed to the PsaA branch of electron transfer. Photoaccumulation at pH 10 and 220 K produces a maximum of four spins per P700(z.rad;+), and proton ENDOR spectra indicate that a second phyllosemiquinone is being photoaccumulated, with markedly different proton hyperfine couplings (hfcs). This phyllosemiquinone is unaffected by mutation of PsaAW693, confirming that it does not arise from the PsaA branch of electron transfer, and we therefore attribute it to the PsaB phyllosemiquinone.

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http://dx.doi.org/10.1016/s0005-2728(02)00281-5DOI Listing

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