In this study the previous finding of similar variable regions in individual IgG1 and IgG2a antibody populations is extended by the demonstration of similar fine specificity of IgG1 and IgG2a combining sites. Antibody populations from individual mice directed against oligo-D-alanine determinants were analyzed in their cross-reactivity towards 5 heterologous dipeptides. This was done by mixing antibody and hapten followed by determination of free antibodies in a kinetic red cells sensitization assay. The comparison of hyperimmune sera from 10 mice showed that genetically identical mice can differ significantly in their cross-reaction pattern. Within each serum the cross-reaction pattern was determined for IgG1 and IgG2a. With a few exceptions the same individual pattern was found in both IgG1 and IgG2a antibody populations. This was taken as evidence that the combining sites of IgG1 and IgG2a anti-oligo-D-alanine antibody populations in an individual mouse are similar.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/eji.1830050110 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An Update on Multi-System Inflammatory Syndrome in Children.
Curr Rheumatol Rep
January 2025
Division of Rheumatology, Department of Pediatrics, The Warren Alpert Medical School of Brown University, 593 Eddy Street, Providence, RI, 02903, USA.
Purpose: To summarize the latest research on the epidemiology, pathogenesis, diagnosis, and treatment of multisystem inflammatory syndrome in children (MIS-C).
Recent Findings: The epidemiology of MIS-C has been dynamic since its initial description. The pathogenesis remains poorly understood.
Direct repeat region 3' end modifications regulate Cas12a activity and expand its applications.
Nucleic Acids Res
January 2025
Guangdong Provincial Key Laboratory of Digestive Cancer Research, Digestive Diseases Center, Scientific Research Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong 518107, P.R. China.
CRISPR-Cas12a technology has transformative potential, but as its applications grow, enhancing its inherent functionalities is essential to meet diverse demands. Here, we reveal a regulatory mechanism for LbCas12a through direct repeat (DR) region 3' end modifications and de-modifications, which can regulate LbCas12a's cis- and trans-cleavage activities. We extensively explored the effects of introducing phosphorylation, DNA, photo-cleavable linker, DNA modifications at the DR 3' end on LbCas12a's functionality.
View Article and Find Full Text PDFThe impact of signaling pathways on the desmosome ultrastructure in pemphigus.
Front Immunol
January 2025
Chair of Vegetative Anatomy, Institute of Anatomy, Faculty of Medicine, Ludwig-Maximilan-Universität (LMU) Munich, München, Germany.
Introduction: The autoantibody-driven disease pemphigus vulgaris (PV) impairs desmosome adhesion in the epidermis. In desmosomes, the pemphigus autoantigens desmoglein 1 (Dsg1) and Dsg3 link adjacent cells. Dsgs are clustered by plaque proteins and linked to the keratin cytoskeleton by desmoplakin (Dp).
View Article and Find Full Text PDFCellular and humoral immune responses in cats vaccinated with feline herpesvirus 1 modified live virus vaccine.
Front Vet Sci
January 2025
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
Feline herpesvirus 1 (FHV-1) is an important pathogen causing infectious rhinotracheitis in felids, mainly infecting the upper respiratory tract and conjunctiva. Multiple vaccines are available to prevent FHV-1 infection, and the antibody levels are always used to evaluate their effectiveness. However, the cellular immunity response following immunization in cats remains unclear.
View Article and Find Full Text PDFGardnerella vaginalis-binding IgA in the urethra of sexually experienced males.
Microbiome
January 2025
Department of Medicine, University of Toronto, Toronto, Canada.
Background: Genital inflammation increases HIV susceptibility and is associated with the density of pro-inflammatory anaerobes in the vagina and coronal sulcus. The penile urethra is a critical site of HIV acquisition, although correlates of urethral HIV acquisition are largely unknown. While Streptococcus mitis is a consistent component of the urethral flora, the presence of Gardnerella vaginalis has been linked with prior penile-vaginal sex and urethral inflammation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!