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Expression Cloning of Antibodies from Single Human B Cells.
Methods Mol Biol
October 2024
Division of B Cell Immunology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
The majority of lymphomas originate from B cells at the germinal center stage. Preferential selection of B-cell clones by a limited set of antigens has been suggested to drive lymphoma development. While recent studies in B-cell chronic lymphocytic leukemia (CLL) have shown that self-reactive B-cell receptors (BCR) can generate cell-autonomous signaling and proliferation, our knowledge about the role of BCRs for the development or survival of other lymphomas remains limited.
View Article and Find Full Text PDFA Novel Method for In Situ RT-PCR Based on Capsules from Centrifuge Tubes, Ideal for Transcripts Detection in Plant Tissues.
Methods Mol Biol
July 2024
Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Merida, Yucatán, Mexico.
In situ RT-PCR presents advantages over other expression analysis methods due to its rapid processing and low-cost equipment. However, this technique is not without its challenges. A protocol based on a capsule made from centrifuge tubes that offers advantages over slides is presented.
View Article and Find Full Text PDFDirect Detection of SARS-CoV-2 RNA in Saliva with Colorimetric RT-LAMP.
Methods Mol Biol
June 2024
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
RT-LAMP is an effective alternative to RT-PCR-based diagnostics, offering high specificity, sensitivity, and rapid results. One notable advantage is the robustness of its enzymes, allowing for direct amplification from crude samples without the need for prior isolation of RNA. Colorimetric LAMP is particularly attractive as it eliminates the need for complex instrumentation, making it suitable for point-of-care applications.
View Article and Find Full Text PDFDetection of Alicyclobacillus spp. and Identification of Guaiacol Production Using the GENE-UP® PRO ACB, IFU Method No. 12, and Cosmo Bio Assays.
J Food Prot
August 2023
Department of Food Science, Cornell University, Ithaca, NY 14853, USA. Electronic address:
Some species of Alicyclobacillus spoil beverages by producing guaiacol. Current culture-based methods detect the presence of Alicyclobacillus spp. and a subsequent peroxidase assay determines if the isolate can produce guaiacol.
View Article and Find Full Text PDFRT-PCR based detection of Pepper mild mottle virus from capsicum seeds and seed transmission assay.
Virusdisease
March 2023
Department of Plant Pathology, CSK HPKV, Palampur, Himachal Pradesh 176062 India.
Unlabelled: Pepper mild mottle virus (PMMoV), a from family, is highly contagious and transmitted by seeds as well as soil in nature. PMMoV has become a greater threat to capsicum cultivation worldwide. To develop an indigenous, rapid, and sensitive protocol for routine detection of PMMoV from seeds, the sensitivity of DAS-ELISA and RT-PCR was compared in the present study.
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