Hydrolysis of a protein mixture from muscle and bone tissues with the enzymatic system from procine pancreatic cell suspension was studied. Kinetic constants and the values of activation energy were determined for individual processes of the release of 15 amino acids. The kinetic characteristics of the overall enzymatic hydrolysis calculated from analysis of the changes in concentrations of terminal amino groups were compared with the characteristics obtained while studying the accumulation patterns of individual amino acids.
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Ecotoxicol Environ Saf
January 2025
Universidad Nacional de San Juan, Facultad de Ingeniería (FI-UNSJ), Av. Lib. San Martín (Oeste) 1109, San Juan, San Juan 5400, Argentina; Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria San Juan, Calle 11 y Vidart, Pocito, San Juan 5427, Argentina. Electronic address:
Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L.
View Article and Find Full Text PDFAnn Surg Oncol
January 2025
Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Republic of Korea.
Background: Three dimensional (3D) cell cultures can be effectively used for drug discovery and development but there are still challenges in their general application to high-throughput screening. In this study, we developed a novel high-throughput chemotherapeutic 3D drug screening system for gastric cancer, named 'Cure-GA', to discover clinically applicable anticancer drugs and predict therapeutic responses.
Methods: Primary cancer cells were isolated from 143 fresh surgical specimens by enzymatic treatment.
Drug Deliv Transl Res
January 2025
Dr. Hari Singh Gour Central University, Sagar, 470003, MP, India.
Cancer treatments such as surgery and chemotherapy have several limitations, including ineffectiveness against large or persistent tumors, high relapse rates, drug toxicity, and non-specificity of therapy. Researchers are exploring advanced strategies for treating this life-threatening disease to address these challenges. One promising approach is targeted drug delivery using prodrugs or surface modification with receptor-specific moieties for active or passive targeting.
View Article and Find Full Text PDFFront Cell Infect Microbiol
January 2025
Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan, China.
Endophytic fungi associated with selected aquatic plants, and were evaluated. sp. nov.
View Article and Find Full Text PDFSe Pu
February 2025
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Chemical modifications are widely used in research fields such as quantitative proteomics and interaction analyses. Chemical-modification targets can be roughly divided into four categories, including those that integrate isotope labels for quantification purposes, probe the structures of proteins through covalent labeling or cross-linking, incorporate labels to improve the ionization or dissociation of characteristic peptides in complex mixtures, and affinity-enrich various poorly abundant protein translational modifications (PTMs). A chemical modification reaction needs to be simple and efficient for use in proteomics analysis, and should be performed without any complicated process for preparing the labeling reagent.
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