AI Article Synopsis
- AoXlnR is a transcriptional activator responsible for regulating the major xylanase gene, xynF1, in the fungus Aspergillus oryzae.
- Researchers conducted a study using Northern blotting to examine the expression levels of various xylanolytic and cellulolytic genes in different strains of A. oryzae, including wild type, a disruptant, and an overexpressed strain of AoXlnR.
- The findings revealed that AoXlnR influences the expression of at least eight genes (four xylanolytic and four cellulolytic), which are activated by xylan and D-xylose, and also that it plays a role in stimulating the expression of these genes
Article Abstract
AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain was analyzed by Northern blotting. AoXlnR mediated expression of at least four xylanolytic genes and four cellulolytic genes when induced by xylan and D-xylose. Moreover, AoXlnR was newly found to mediate the cellulose-inductive expression of the xylanolytic genes as well as the cellulolytic genes.
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| http://dx.doi.org/10.1016/s0014-5793(02)03328-8 | DOI Listing |
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