A peptide named apisimin was found in honeybee (Apis mellifera L.) royal jelly (RJ). N-terminal sequencing showed that this peptide corresponded to the sequence of a cDNA clone isolated from an expression cDNA library prepared from heads of nurse honeybees. No homology was found between the protein sequence of apisimin with a molecular mass of 5540.4 Da and sequences deposited in the Swiss-Prot database. The 54 amino acids of apisimin do not include Cys, Met, Pro, Arg, His, Tyr, and Trp residues. The peptide shows a well-defined secondary structure as observed by CD spectroscopy, and has the tendency to form oligomers. Isoelectrofocusing showed apisimin to be an acidic peptide.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0014-5793(02)03272-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bee reasonable: Do bumblebees reason by exclusion?
Learn Behav
January 2025
ICREA, Pg. Lluís Companys 23, Barcelona, Spain.
In a clever adaptation of the two-cups task, a recent paper tested for reasoning by exclusion in bees. Although further work is necessary to rule out competing hypotheses, this study advances our ability to test cognitive capacities in invertebrates.
View Article and Find Full Text PDFSafety of 1 mcg/mL as the starting dose in cluster protocol for hymenoptera immunotherapy.
Allergol Immunopathol (Madr)
January 2025
Department of Allergy and Clinical Immunology, Firooz Abadi Hospital, Iran University of Medical Sciences, Tehran, Iran;
Background: Hymenoptera venom allergy is a potentially severe allergic reaction in the general population. The only preventative approach in these cases is venom immunotherapy (VIT), which follows different protocols. The recommended initial dose is 0.
View Article and Find Full Text PDFRecycling honey bee drone brood for sustainable beekeeping.
J Econ Entomol
December 2024
Institute of Bioanalytics and Agro-Metabolomics, Department of Agrobiotechnology IFA-Tulln, University of Natural Resources and Life Sciences Vienna (BOKU), Tulln, Austria.
Pollination by insects is vital for global agriculture, with honey bees (Apis mellifera L.) being the most important pollinators. Honey bees are exposed to numerous stressors, including disease, pesticides, and inadequate nutrition, resulting in significant colony losses.
View Article and Find Full Text PDFLethal and sublethal effects of flupyradifurone and cyantraniliprole on two neotropical stingless bee species.
Ecotoxicology
January 2025
Tecnológico Nacional de México, Instituto Tecnológico de Conkal, Conkal, Yucatán, Mexico.
Stingless bees are important pollinators in tropical regions, but their survival and behavior have been impacted by various factors, including exposure to insecticides. Here, we evaluated the lethal and sublethal effects of commercial formulations of two widely used insecticides, flupyradifurone (FPF formulation), and cyantraniliprole (CY formulation), on Melipona beecheii and Nannotrigona perilampoides. The study involved oral exposure of bees to insecticides, calculation of the lethal concentration (LC) and the lethal time (LT), and evaluation of walking and flight take-off activities.
View Article and Find Full Text PDFMolecular detection of the zoonotic trematode Centrocestus formosanus (Nishigori, 1924) (Opisthorchiida, Heterophyidae) in Central Europe.
Vet Res Commun
January 2025
Department of Clinical Sciences and Translational Medicine, Faculty of Medicine, University of Rome "Tor Vergata", Via Montpellier 1, Rome, 001 33, Italy.
In our study, fancy southern platyfish Xiphophorus maculatus (Cyprinodontiformes, Poeciliidae) were examined due to breathing disorders and mortality. Fish came from Vietnam farm and were redistributed by international wholesaler. In fish, loss of appetite and gasping near the water surface was observed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!