Background: Human sarcomas have a propensity for aggressive local invasion and early pulmonary metastasis. Frequently, deaths are due to uncontrolled pulmonary metastases. The purpose of the current study was to evaluate cytogenetics, tumorigenicity, metastatic potential, and production of angiogenic factors in human sarcoma cell strains. A secondary purpose was to establish low passage cell strains for studying new therapeutic approaches.
Methods: The authors established 11 cell strains from human sarcoma surgical specimens and characterized their in vitro tumor properties, including growth in soft agar, expression of angiogenic growth factors (vascular endothelial growth factor [VEGF] and basic-fibroblast growth factor [bFGF]), and cytogenetics.
Results: All of the cell strains remained diploid. All exhibited the ability to grow in soft agar and expressed both VEGF as well as bFGF. In addition, 6 of the 11 established sarcoma cell strains were tumorigenic, 5 of which spontaneously metastasized to the lungs in nude mice. Four of the five cell strains that yielded lung metastases were derived from lung metastases in patients.
Conclusions: The 11 cell strains, which were derived from diverse sarcoma histologies, will provide a model for studying not only metastatic progression but also the in vitro and in vivo efficacy of new therapeutic modalities for human sarcomas.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/cncr.10879 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Correlative Raman-Voltage Microscopy Revealing the Localized Structure-Stress Relationship in Silicon Solar Cells.
ACS Nano
January 2025
Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123, P. R. China.
Knowledge of localized strain at the micrometer scale is essential for tailoring the electrical and mechanical properties of ongoing thinning of crystal silicon (c-Si) solar cells. Thinning c-Si wafers below 110 m are susceptible to cracking in manufacturing due to the nonuniform stress distribution at a micrometer region, necessitating a rigorous technique to reveal the localized stress distribution correlating with its device electrical output. In this context, a Raman microscopy integrated with a photovoltage mapping setup with high resolution to the submicrometer scale is developed to acquire correlative Raman-voltage of the localized physical properties at the microcracks on the rear side of c-Si solar cells.
View Article and Find Full Text PDFDevelopment and characterization of a reverse genetics system for the lineage II Chicava strain of Machupo virus in a guinea pig model.
PLoS Negl Trop Dis
January 2025
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, United States of America.
Background: Machupo virus (MACV) is a New World mammarenavirus (hereafter referred to as "arenavirus") and the etiologic agent of Bolivian hemorrhagic fever (BHF). No vaccine or antiviral therapy exists for BHF, which causes up to 35% mortality in humans. New World arenaviruses evolve separately in different locations.
View Article and Find Full Text PDFHuman naïve B cells recognize prepandemic influenza virus hemagglutinins.
Sci Immunol
January 2025
Ragon Institute of Mass General, MIT, and Harvard, Cambridge, MA 02139, USA.
Understanding the naïve B cell repertoire and its specificity for potential zoonotic threats, such as the highly pathogenic avian influenza (HPAI) H5Nx viruses, may allow prediction of infection- or vaccine-specific responses. However, this naïve repertoire and the possibility to respond to emerging, prepandemic viruses are largely undetermined. Here, we profiled naïve B cell reactivity against a prototypical HPAI H5 hemagglutinin (HA), the major target of antibody responses.
View Article and Find Full Text PDFElucidation of the Glycan Structure of the b-type Flagellin of PAO1.
ACS Infect Dis
January 2025
Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden 2333 ZA, The Netherlands.
Flagella are essential for motility and pathogenicity in many bacteria. The main component of the flagellar filament, flagellin (FliC), often undergoes post-translational modifications, with glycosylation being a common occurrence. In PAO1, the b-type flagellin is -glycosylated with a structure that includes a deoxyhexose, a phospho-group, and a previous unknown moiety.
View Article and Find Full Text PDFThe Viral K1 Killer Yeast System: Toxicity, Immunity, and Resistance.
Yeast
January 2025
Department of Genetics, Stanford University, Stanford, California, USA.
Killer yeasts, such as the K1 killer strain of S. Cerevisiae, express a secreted anti-competitive toxin whose production and propagation require the presence of two vertically-transmitted dsRNA viruses. In sensitive cells lacking killer virus infection, toxin binding to the cell wall results in ion pore formation, disruption of osmotic homeostasis, and cell death.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!