DNA X-irradiated in oxygenated aqueous solution produces the formamido lesion from the breakdown of pyrimidine nucleosides. This pyrimidine breakdown product inhibits the hydrolysis by nuclease P1 of the phosphoester bond 3' to the damaged nucleoside. Consequently, the lesion can be obtained from an enzymatic digest of the DNA as a modified dinucleoside monophosphate in which the 5' nucleoside contains the lesion. In this form, the formamido lesion can be detected with good sensitivity by liquid chromatography-mass spectrometry (LC-MS). Nucleosides that have lost the base moiety also inhibit nuclease P1. Together, the formamido and abasic lesions account for all of the substantial peaks in the LC-MS ion current profile.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1667/0033-7587(2002)158[0538:dacofl]2.0.co;2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of the exposure to perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) through drinking water and human risk assessment.
J Environ Health Sci Eng
June 2025
Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran.
In the present study, two most commonly used Perfluoroalkyl substances (PFASs), namely perfluorooctanoic acid (PFOA) and perfluorooctanesulfonate (PFOS), were determined in 45 tap water samples from the city of Isfahan (Iran) by dispersive liquid-liquid extraction (DLLME) and liquid chromatography-mass spectrophotometry (LC-MS) analysis. Risk assessment was also performed to determine the risk to human health. The mean concentration of PFOA was 38.
View Article and Find Full Text PDFChlorine Dioxide Dry Gas: A Promising Solution for Effective Decontamination of Cephalosporin Compounds.
Appl Biosaf
December 2024
Royal Society of Biology, London, United Kingdom.
Introduction: Cephalosporins can trigger hypersensitivity reactions in certain individuals. Consequently, strict regulations restrict the production of non-beta-lactam substances during or after cephalosporin manufacturing. Dry chlorine dioxide gas (dClO), together with ultra-performance liquid chromatography Mass spectrometry/mass spectrometry (UPLC-MS/MS) detection methods, has emerged as a promising method for decontaminating cephalosporin compounds.
View Article and Find Full Text PDFDevelopment of a liquid chromatography-mass spectrometry based targeted metabolomics method for discovering diagnostic biomarkers in Kawasaki disease.
J Chromatogr A
December 2024
Shanghai Center for Clinical Laboratory, Shanghai, PR China. Electronic address:
Kawasaki disease (KD) has emerged as the leading cause of acquired heart disease in children, primarily due to the absence of highly sensitive and specific biomarkers for early and accurate diagnosis. To address this issue, a simple and comprehensive targeted metabolomics method employing ultra high-performance liquid chromatography coupled with Q-TRAP mass spectrometry has been developed to identify new metabolite biomarkers for KD. This method enables the simultaneous quantification of 276 metabolites, covering 60 metabolic pathways, with a particular emphasis on metabolites relevant to KD.
View Article and Find Full Text PDFProteomic Analysis of Anticancer Effect of Myo-inositol in Human Prostate Cancer (DU-145) Cell Line.
Asian Pac J Cancer Prev
December 2024
Department of Molecular Biology, EW Villa Medica, Dhaka, Bangladesh.
Objective: This study investigated the potential anticancer properties of Myo-inositol on the DU-145 prostate cancer cell line.
Methods: The DU-145 cells have been treated to different doses of Myo-inositol in order to ascertain the half-maximal inhibitory concentration (IC50) using the trypan blue exclusion assay. The impact of Myo-inositol on proteomic profiles was evaluated using 2D gel electrophoresis and liquid chromatography-mass spectrometry (LC-MS).
OligoDistiller: A Platform Agnostic Software Tool for MS and MS Data Analysis of Complex Oligonucleotides and Their Impurities.
Anal Chem
December 2024
Janssen Pharmaceutica N.V., Turnhoutseweg 30, 2340 Beerse, Belgium.
Oligonucleotides are currently one of the most rapidly advancing classes of therapeutic modalities. Understanding critical quality attributes, such as the impurity profile, stability, potential metabolites, and sequence conformity, is the key to their ultimate success. To obtain the information presented above, liquid chromatography-mass spectrometry (LC-MS) is often employed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!