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VE-PTP and VE-cadherin ectodomains interact to facilitate regulation of phosphorylation and cell contacts. | LitMetric

AI Article Synopsis

  • VE-cadherin is a key adhesion molecule in endothelial cells, and the regulation of its phosphorylation is vital for maintaining adherens junction integrity.
  • The study found that VE-PTP, a receptor-type phosphatase, binds to VE-cadherin without interacting with beta-catenin, needing specific extracellular domains for this co-precipitation.
  • Interestingly, VE-PTP can reduce the phosphorylation of VE-cadherin and improve cell layer integrity without requiring its enzymatic activity, indicating a novel role as a binding partner for VE-cadherin.

Article Abstract

VE-cadherin is the essential adhesion molecule in endothelial adherens junctions, and the regulation of protein tyrosine phosphorylation is thought to be important for the control of adherens junction integrity. We show here that VE-PTP (vascular endothelial protein tyrosine phosphatase), an endothelial receptor-type phosphatase, co-precipitates with VE-cadherin, but not with beta-catenin, from cell lysates of transfected COS-7 cells and of endothelial cells. Co-precipitation of VE-cadherin and VE-PTP required the most membrane-proximal extracellular domains of each protein. Expression of VE-PTP in triple-transfected COS-7 cells and in CHO cells reversed the tyrosine phosphorylation of VE-cadherin elicited by vascular endothelial growth factor receptor 2 (VEGFR-2). Expression of VE-PTP under an inducible promotor in CHO cells transfected with VE-cadherin and VEGFR-2 increased the VE-cadherin-mediated barrier integrity of a cellular monolayer. Surprisingly, a catalytically inactive mutant form of VE-PTP had the same effect on VE-cadherin phosphorylation and cell layer permeability. Thus, VE-PTP is a transmembrane binding partner of VE-cadherin that associates through an extracellular domain and reduces the tyrosine phosphorylation of VE-cadherin and cell layer permeability independently of its enzymatic activity.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC126293PMC
http://dx.doi.org/10.1093/emboj/cdf497DOI Listing

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