AI Article Synopsis
- The study investigated how various factors like pH, temperature, and additives influenced the production of phytase by the newly identified bacterium Mitsuokella jalaludinii from cattle rumen.
- The research found that sodium phytate increased phytase production, while glucose in the medium was inhibitory.
- Ideal conditions for phytase production were determined to be a pH of 7.02 and a temperature of 39°C, and the presence of surfactants did not impact production levels.
Article Abstract
Aims: The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated.
Method And Results: By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by sodium phytate. Inclusion of glucose into rice bran-soybean milk (RB-SM) medium inhibited the phytase production. Supplementation of 0.05-0.5% of phosphate to RB-SM medium neither inhibited nor stimulated the phytase production. The optimum pH and temperature for the phytase production of M. jalaludinii were 7.02 and 39 degrees C, respectively. Surfactants such as Tween-80 (0.5% and 1.0%) and Triton X-100 (1.0%) did not affect the phytase production.
Conclusions, Significance And Impact Of The Study: The study has provided important data on the culture conditions affecting phytase production of M. jalaludinii, which will facilitate future research on the optimization of fermentation processes for production of high phytase activity by M. jalaludinii.
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| http://dx.doi.org/10.1046/j.1365-2672.2002.01727.x | DOI Listing |
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