The two chain "Insulin/Insulin-like Growth Factor-I" hybrids, Ins/IGF-I(8) and Ins/IGF-I(11), were obtained by means of enzymatic semisynthesis, using desoctapeptide insulin (DOI) and the octapeptide and undecapeptide chemically synthesized according to the sequence 22-29 and 22-32 of IGF-I respectively as the starting materials. Comparative studies of the molecules with insulin indicate that the hybrid molecules retain in vivo the full activity of insulin. So the replacement of B27Thr by Asn and B30Ala by Thr, and the exchange of the sequence orders of B25 and B26, B28 and B29, as well as the extension of tripeptide (Gly-Tyr-Gly) at the B30 do not affect insulin activity.
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