AI Article Synopsis
- The study assessed how metal ions (Fe2+, Cu2+, Zn2+) impact the effectiveness of epigallocatechin gallate (EGCG) in protecting liver cells from bromobenzene-induced damage.
- When tested alone, EGCG (0.02 mM) did not protect the cells, but it was effective when combined with zinc (0.02 mM), indicating the significance of zinc in enhancing EGCG's protective effects.
- The research confirmed that EGCG forms a complex with zinc through UV-VIS absorption spectroscopy, highlighting this interaction's role in boosting EGCG's hepatoprotective activity.
Article Abstract
The influence of metal ions (Fe2+, Cu2+, Zn2+) on the hepatoprotective activity of epigallocatechin gallate (EGCG) against hepatotoxin-induced cell injury was investigated. Primary cultures of rat hepatocytes were treated with a well-known hepatotoxin, bromobenzene (BB), in the presence of EGCG only or EGCG plus each metal ion. After 24 h, 0.02 mM EGCG did not show protective activity on the cultured hepatocytes. In contrast, the hepatocytes were protected against BB in the presence of 0.02 mM EGCG and 0.02 mM zinc. The addition of only zinc could not protect hepatocytes against BB. These results suggest that the formation of the zinc-EGCG complex is very important in the enhancement of the hepatoprotective activity of EGCG. The complexation of EGCG with zinc was confirmed by UV-VIS absorption spectroscopy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1248/bpb.25.1156 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!