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Fast and accurate multi-bacterial identification using cleavable and FRET-based peptide nucleic acid probes.
Biosens Bioelectron
March 2025
Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea; Center for Genomic Integrity, Institute for Basic Science, Ulsan, 44919, Republic of Korea. Electronic address:
Fast and accurate identification of pathogenic microbes in patient samples is crucial for the timely treatment of acute infectious diseases such as sepsis. The fluorescence in situ hybridization (FISH) technique allows the rapid detection and identification of microbes based on their variation in genomic sequence without time-consuming culturing or sequencing. However, the recent explosion of microbial genomic data has made it challenging to design an appropriate set of probes for microbial mixtures.
View Article and Find Full Text PDFCorrelation of sperm motility, acrosome integrity, protamine deficiency, and DNA fragmentation in proven and unproven Friesian Holstein bulls.
J Adv Vet Anim Res
September 2024
Research Center for Applied Zoology, National Research and Innovation Agency, Bogor, Indonesia.
Article Synopsis
- This study evaluates the frozen semen quality of Friesian Holstein bulls to assess male fertility for artificial insemination, comparing proven and unproven bulls based on various scientific analyses.
- Six proven and six unproven bulls' semen was tested using advanced techniques like Computer Assisted Semen Analysis and staining methods to measure parameters like sperm motility, viability, and DNA integrity.
- Results showed no significant differences in frozen semen quality between proven and unproven bulls, indicating that both types are equally viable for breeding programs and highlighting the importance of molecular analysis in assessing semen health.
Synthetic Protein-to-DNA Input Exchange for Protease Activity Detection Using CRISPR-Cas12a.
Anal Chem
November 2024
Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area Delle Scienze 17/A, 43124 Parma, Italy.
We present a novel activity-based detection strategy for matrix metalloproteinase 2 (MMP2), a critical cancer protease biomarker, leveraging a mechanism responsive to the proteolytic activity of MMP2 and its integration with CRISPR-Cas12a-assisted signal amplification. We designed a chemical translator comprising two functional units─a peptide and a peptide nucleic acid (PNA), fused together. The peptide presents the substrate of MMP2, while the PNA serves as a nucleic acid output for subsequent processing.
View Article and Find Full Text PDFSupramolecular multiplexes from collagen mimetic peptide-PNA(GGG) conjugates and C-rich DNA: pH-induced reversible switching from triplex-duplex to triplex--motif.
Biomater Sci
December 2024
Department of Chemistry, Indian Institute of Science Education and Research (IISER) Tirupati, Karkambadi Road, Mangalam, Tirupati 517507, India.
Peptides are well known for forming nanoparticles, while DNA duplexes, triplexes and tetraplexes create rigid nanostructures. Accordingly, the covalent conjugation of peptides to DNA/RNA produces hybrid self-assembling features and may lead to interesting nano-assemblies distinct from those of their individual components. Herein, we report the preparation of a collagen mimetic peptide incorporating lysine in its backbone, with alkylamino side chains radially conjugated with G-rich PNA [collagen-(PNA-GGG)].
View Article and Find Full Text PDFApplication of FISH based G2-PCC assay for the cytogenetic assessment of high radiation dose exposures: Potential implications for rapid triage biodosimetry.
PLoS One
October 2024
Cytogenetic Biodosimetry Laboratory, Radiation Emergency Assistance Center/Training Site, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, Oak Ridge, Tennessee, United States of America.
The main goal of this study is to test the utility of calyculin A induced G2-PCC assay as a biodosimetry triage tool for assessing a wide range of low and acute high radiation dose exposures of photons. Towards this initiative, chromosome aberrations induced by low and high doses of x-rays were evaluated and characterized in G2-prematurely condensed chromosomes (G2-PCCs) by fluorescence in situ hybridization (FISH) using human centromere and telomere specific PNA (peptide nucleic acid) probes. A dose dependent increase in the frequency of dicentric chromosomes was observed in the G2-PCCs up to 20 Gy of x-rays.
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