The study presented here examined the efficacy of a commercially available qualitative immunochromatographic assay for detecting Cryptosporidium oocysts in stool samples. A total of 75 samples were tested, including 50 positive for Cryptosporidium spp. by acid-fast stain, 20 positive for other parasites ( Blastocystis hominis, Endolimax nana, Entamoeba coli, Giardia lamblia, Ascaris lumbricoides, Strongyloides stercoralis and Trichuris trichiura), and five negative samples. The observed sensitivity was 98%, while specificity was 100%; the detection threshold was near 1000 oocysts/ml. Correctly diagnosed positive samples included Cryptosporidium parvum genotypes 1 and 2, whereas the single false-negative sample corresponded to a Cryptosporidium meleagridis infection.
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http://dx.doi.org/10.1007/s10096-002-0778-1 | DOI Listing |
Water Environ Res
January 2025
Laboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo (CIAD), Culiacán, Sinaloa, Mexico.
The reliance on agriculture in many nations has increased the use of treated wastewater for irrigation. However, reclaimed water still poses health risks from resistant pathogens like Cryptosporidium spp. Ozone, a strong disinfectant, has been used in water treatment.
View Article and Find Full Text PDFVet Parasitol Reg Stud Reports
January 2025
Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address:
Cryptosporidium is a leading cause of diarrhea in children and immunocompromised patients. Various animals and birds can also be infected with this protist, and Cryptosporidium zoonosis is common. A few reports have been published worldwide on Cryptosporidium infections in chickens.
View Article and Find Full Text PDFAMB Express
January 2025
Parasitology and Animal Diseases Department, Veterinary Research Institute, National Research Centre, El Buhouth St., Dokki, Giza, Egypt.
Cryptosporidium sp. is an obligatory intracellular apicomplexan protozoan parasite that causes a disease called cryptosporidiosis with substantial veterinary and medical importance. Therefore, this study aimed to evaluate an early diagnosis of cryptosporidiosis using the anti-Cryptosporidium parvum oocyst immunoglobulin IgG polyclonal antibodies (anti-C.
View Article and Find Full Text PDFInt J Vet Sci Med
January 2025
Galicia (Grupo INVESAGA). Departamento de Patología Animal. Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain, Investigación en Sanidad Animal.
Although , and some species are frequently involved in neonatal calf diarrhoea (NCD), detailed studies on their interactions are scarce. Therefore, a cross-sectional study including faecal samples from 404 diarrhoeic calves aged 0-30 days was performed. oocysts and cysts were detected by immunofluorescence antibody test and positive samples were molecularly characterized.
View Article and Find Full Text PDFFront Vet Sci
December 2024
Department of Zoology, Abdul Wali Khan University, Mardan, Pakistan.
Background: spp. are protozoan parasites that infect the gastrointestinal tract of various animals, including goats, and can also contaminate water sources, posing a significant public health risk. Detecting oocysts in fecal and water samples is critical for understanding the epidemiology of cryptosporidiosis and implementing appropriate control measures.
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