The aim of this study was to develop a culture method for Toxoplasma gondii that could provide fresh viable tachyzoites as and when required. When T. gondii was continuously maintained in HeLa cell cultures at 37 degrees C, the time to harvest varied from 48 to 144 h. Tachyzoite yields of > or = 1 x 10(6)/ml and > or = 90% viable were obtained from 519/882 (58.8%) cultures and 120/155 (77.4%) harvests were successfully used in the dye test. When cultures were transferred from 37 to 25 degrees C when maximally infected (48-54-h post-infection), they could be stabilised and tachyzoites could be harvested as required, up to 168 h later. When harvested from 25 degrees C, significantly more cultures 783/811 (96.5%) produced tachyzoite yields > or = 1 x 10(6)/ml > or = 90% viable (p < 0.001). Tachyzoite quality also significantly improved and 206/224 harvests (91.9%) (p < 0.001) were successfully used in the dye test. We have demonstrated that tachyzoites can be maintained at dye test quality for at least 7 days in HeLa cultures at 25 degrees C. The system is flexible and robust and provides a means whereby tachyzoites of standard quality can be stored for use in experimental models as and when required.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0167-7012(02)00101-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fabrication of novel multifunctional copper-functionalized hemp fibers to remove anionic dye and non-steroidal anti-inflammatory drugs from wastewaters.
Chemosphere
January 2025
Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Salerno, Italy.
This research aims to design a novel selective and multifunctional adsorbent based on Al/Cu modified hemp fibres as a novel and multifunctional adsorbent for removing different classes of pollutants. The adsorbent, which was widely characterized, was shown to be more effective in removing anionic dyes compared to cationic ones. Among the tested dye, methyl orange was selected to understand how different parameters, such as temperature (20-80°C), contact time, pH (2-12), initial dye concentration (50-300 ppm), salinity and adsorbent dosage (1-10 g/L) affect the removal capacity.
View Article and Find Full Text PDFSynthesizing hybrid copper phosphate (Cu(PO)) nanoflowers using Cu and shed snakeskin: antioxidant, antibacterial, anticancer, guaiacol, anionic, and cationic dye degradation properties.
J Biol Eng
January 2025
Department of Aquatic Animals and Diseases, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Türkiye.
Background: Synthesis of organic@inorganic hNFs is achieved by the coordination of organic compounds containing amine, amide, and diol groups with bivalent metals. The use of bio-extracts containing these functional groups instead of expensive organic inputs such as DNA, enzymes, and protein creates advantages in terms of cost and applicability. In this study, the application potentials (antioxidant, antibacterial, anticancer, guaiacol, anionic, and cationic dye degradation) of hybrid (organic@inorganic) nanoflowers (hNFs) synthesized with Cu and snakeskin (SSS) were proposed.
View Article and Find Full Text PDFCharacterization and evaluation of the immobilized laccase enzyme potential in dye degradation via one factor and response surface methodology approaches.
Sci Rep
January 2025
Faculty of Biotechnology, German International University, Regional Ring Road, East Cairo, New Administrative Capital, Cairo, Egypt.
In the current study, calcium alginate was used as a carrier for Agaricus bisporus CU13 laccase immobilization, with an immobilization yield of the entrapped laccase of 91.95%. Free and immobilized enzymes showed their best enzyme activity at 60 °C as an optimum temperature.
View Article and Find Full Text PDFIncorporation of opacifier to Type III alpha gypsum and its effect on scannability and linear setting expansion - An in vitro study.
J Indian Prosthodont Soc
January 2025
Department of Prosthodontics and Crown and Bridge, Sri Sai College of Dental Surgery, Vikarabad, Telangana, India.
Aim: This study aims to enhance the scannability of Type III alpha gypsum by incorporating an opacifier and to evaluate its effect on the LSE property.
Setting And Design: In vitro - Comparative study.
Materials And Methods: The base powder of Type III alpha gypsum was divided into three groups: Group I (100 g of base powder), Group II (90 g of base powder with 10 g of TiO2), and Group III (80 g of base powder with 20 g of TiO2).
Comparison of the antibacterial efficacy of 810 nm diode laser and photodynamic therapy in reducing microbial flora in patients with peri-implant mucositis - An in vivo study.
J Indian Prosthodont Soc
January 2025
Department of Microbiology, Faculty of Medicine and Health Sciences, SGT University, Gurugram, Haryana, India.
Aim: The aim of this study was to investigate and compare the antimicrobial effects of an 810-nanometer diode laser, utilizing or not utilizing toluidine blue as a photosensitizer, in the management of peri-implant mucositis.
Settings And Design: The present study was carried out in 30 implant sites in 15 patients with peri-implant mucositis with a specific inclusion and exclusion criteria. 15 sites were treated utilizing a diode laser (control group) and 15 with photodynamic therapy (test group) in a split-mouth format.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!