[Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized specific monoclonal antibody].

Objective: To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library.

Methods: By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.

Results: Twelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5.

Conclusions: HCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.