A Method of the Simultaneous Purification of Cardiac Troponin T and I.

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)

Department of Cardiology, Zhujiang Hospital, The First Military Medical College, Guangzhou 510280, China.

Published: January 1997

A method of the simultaneous purification of cardiac troponin T (cTnT) and troponin I (cTnI) from human cardiac left ventricular muscle have been developed. Five mg cTnT and 10.2 mg of cTnI were obtained from 100 mg of cardiac muscle. The purity of cTnT and cTnI could reach to 97.6% and 97.2% respectively. Their immunoactivity and specificity have been identified by ELISA method.

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