AI Article Synopsis
- The nucleotide sequence of a 2,387 bp segment from Rhodobacter sphaeroides has been fully determined, which includes the gltD gene responsible for the small subunit of glutamate synthase along with its flanking regions.
- An open reading frame of 1,242 base pairs was identified as the gltD gene, predicting a molecular weight of approximately 44 kD for the protein.
- Comparative analysis showed significant similarities between the gltD sequences from R. sphaeroides, Azospirillum brasilense, and Escherichia coli, particularly noting that R. sphaeroides GltD shares high similarity with A. brasilense GltD, and the gltD-lac
Article Abstract
We have determined the complete nucleotide sequence of a 2 387 bp chromosomal SalI-EcoRI fragment, which contains the structural gene (gltD) for the small subunit of Rhodobacter sphaeroides glutamate synthase, as well as the 5'- and 3'-flanking regions. An open reading frame of 1 242 base pairs was identified as the R. sphaeroides gltD gene. The MW of the small subunit, as deduced from the nucleotide sequence, was estimated to be 44 kD. A comparison of the nucleotide sequence revealed a high similarity among gltD genes of R. sphaeroides, Azospirillum brasilense and Escherichia coli. The deduced amino acid sequence of R. sphaeroides GltD showed a high similarity with that of A. brasilense GltD. The analysis of the binding domains of R. sphaeroides GltD was also carried out. The gltD-lacZ fusion was observed in the presence of 15 mM leucine.
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