AI Article Synopsis
- * This enzyme features a unique "pancreatic loop" that differs structurally from similar loops in pancreatic PLA(2), indicating variability among these proteins.
- * The study reveals the first structure of a PLA(2)-type cardiotoxin, exploring its cardiotoxic and myotoxic activity sites and describing the observed oligomer in its crystalline form.
Article Abstract
The crystal structure of an acidic phospholipase A(2) from Ophiophagus hannah (king cobra) has been determined by molecular replacement at 2.6-A resolution to a crystallographic R factor of 20.5% (R(free)=23.3%) with reasonable stereochemistry. The venom enzyme contains an unusual "pancreatic loop." The conformation of the loop is well defined and different from those in pancreas PLA(2), showing its structural variability. This analysis provides the first structure of a PLA(2)-type cardiotoxin. The sites related to the cardiotoxic and myotoxic activities are explored and the oligomer observed in the crystalline state is described.
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| http://dx.doi.org/10.1016/s1047-8477(02)00022-9 | DOI Listing |
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