Three naphthalene glycosides (1-3), four flavonoids (4-7), and two galloyl glycosides (8-9) were isolated from the stem-bark of Juglans mandshurica (Juglandaceae). Their structures were determined by chemical and spectral means, including to 2D-NMR (COSY, HMQC, and HMBC) experiments. Amongst the isolated compounds, taxifolin (4) showed the most potent HIV-induced cytopethic activity against MT-4 cells with complete inhibitory concentration (IC100) value of 25 microg/ml and maximum cytotoxic concentration (CC100) value of above 100 microg/ml. However, naphthalene glycosides (1-3), flavonoids (5-7), and galloyl tannins (8-9) were inactive against anti-HIV-1 activity.
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