AI Article Synopsis
- Developed high-performance capillary DNA sequencing instruments using a new multicolor fluorescent detection technology.
- The technology features multilaser excitation for fluorescence and "color-blind" single-photon detection for better accuracy.
- Discussed design, testing results of single lane sequencers, and plans for scaling to multilane models.
Article Abstract
We have developed a family of high-performance capillary DNA sequencing instruments based on a novel multicolor fluorescent detection technology. This technology is based on two technical innovations: the multilaser excitation of fluorescence of labeled DNA fragments and the "color-blind" single-photon detection of modulated fluorescence. Our machines employ modern digital and broadband techniques that are essential for achieving superior instrument performance. We discuss the design and testing results for several versions of the automated single lane DNA sequencers, as well as our approach to scaling up to multilane instruments.
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| http://dx.doi.org/10.1002/1522-2683(200208)23:16<2804::AID-ELPS2804>3.0.CO;2-9 | DOI Listing |
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