Leishmania mexicana causes a wide spectrum of clinical diseases. In spite of the variety of clinical forms, no data exist regarding genetic polymorphism of L. mexicana. We analyzed the polymorphism of the internal transcribed spacer (ITS) and the small subunit rRNA genes of 3 reference strains and 24 Mexican isolates of L. mexicana, by means of polymerase chain reaction and subsequent digestion by restriction enzymes. All strains of L. mexicana had invariant patterns for both the ITS and the small subunit of rRNA genes. Leishmania amazonensis and Leishmania venezuelensis displayed polymorphism only in the ITS. The high degree of identity of this region was confirmed by sequencing DNA from three L. mexicana isolates. There was almost complete identity of the sequence for the ITS region of L. venezuelensis and that of strains of Leishmania major, suggesting that these species may be more closely related than previously thought.
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http://dx.doi.org/10.1007/s00436-002-0672-x | DOI Listing |
Plant Biotechnol J
January 2025
Center for Plant Water-use and Nutrition Regulation and College of JunCao Science and Ecology, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou, China.
Heat stress significantly impacts global rice production, highlighting the critical need to understand the genetic basis of heat resistance in rice. U2AF (U2 snRNP auxiliary factor) is an essential splicing complex with critical roles in recognizing the 3'-splice site of precursor messenger RNAs (pre-mRNAs). The U2AF small subunit (U2AF35) can bind to the 3'-AG intron border and promote U2 snRNP binding to the branch-point sequences of introns through interaction with the U2AF large subunit (U2AF65).
View Article and Find Full Text PDFParasitol Int
January 2025
Departamento de Biologia Animal, ICBS, UFRRJ, BR-465 km 7, 23897-000 Seropédica, RJ, Brazil. Electronic address:
Tyzzeria Allen, 1936 is a coccidian genus traditionally associated with Anseriformes, although there are descriptions of Tyzzeria spp. from junglefowls, snakes, skinks and mice. In this sense, the current study performs a taxonomic review of Tyzzeria spp.
View Article and Find Full Text PDFGenes Cells
January 2025
Advanced Biological Information Research Division, INAMORI Frontier Research Center, Kyushu University, Fukuoka, Japan.
Preimplantation embryonic development is orchestrated by dynamic changes in the proteome and transcriptome, regulated by mechanisms such as maternal-to-zygotic transition. Here, we employed label-free quantitative proteomics to comprehensively analyze proteome dynamics from germinal vesicle oocytes to blastocysts in mouse embryos. We identified 3490 proteins, including 715 consistently detected across all stages, revealing stage-specific changes in proteins associated with translation, protein modification, and mitochondrial metabolism.
View Article and Find Full Text PDFZhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
July 2024
Nanchang Municipal Center for Disease Control and Prevention, Base of National Key Laboratory for the Prevention and Control of Infectious Diseases, Nanchang, Jiangxi 330038, China.
Objective: To investigate the prevalence of infection and the distribution of parasite species and genotypes among HIV-positive individuals in Jiangxi Province.
Methods: HIV-positive individuals' sociodemographic and clinical data were collected from three AIDS designated hospitals in Jiangxi Province from January 2022 to March 2023. Subjects' stool samples were collected, and genomic DNA was extracted from stool samples.
Malar J
January 2025
Department of Pharmacology, Kangwon National University School of Medicine, Chuncheon, 24341, Republic of Korea.
Background: The Plasmodium proteasome emerges as a promising target for anti-malarial drug development due to its potential activity against multiple life cycle stages.
Methods: In this investigation, a comparative analysis was conducted on the structural features of the β5 subunit in the 20S proteasomes of both Plasmodium and humans.
Results: The findings underscore the structural diversity inherent in both proteasomes.
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