Objective: To establish a HPCE method to determine the contents of alkalods in Rhizoma Coptidis.
Method: HPCE.
Result: The constituents thus determined have good linearity and separation. The average recovery of coptisine, berberine, palmatine and jatrorrhizine was 98.28%, 98.38%, 97.78% and 98.89% respectively and RSD 0.86%, 1.12%, 1.06% and 1.11% respectively.
Conclusion: The method is convenient, rapid, accurate and practicable. The compatibility reduces the contents of alkaloids in Rhizoma Coptidis in different degrees.
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