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Exopolygalacturonate lyase from Thermotoga maritima: cloning, characterization and organic synthesis application. | LitMetric

Exopolygalacturonate lyase from Thermotoga maritima: cloning, characterization and organic synthesis application.

Carbohydr Res

Unité de Recherches en Biocatalyse (FRE-CNRS 2230), Faculté des Sciences et des Techniques, 2, rue de la Houssinière, BP 92208, F-44322, Nantes, France.

Published: September 2002

AI Article Synopsis

Article Abstract

A new exopolygalacturonate lyase (Pel) gene of the hyperthermophilic bacterium Thermotoga maritima was cloned and overexpressed in Escherichia coli cells. A 42 kDa monomeric Pel was shown to undergo N-terminal processing by cleavage at a putative site between alanine and serine residues. The enzyme catalyzes selectively a beta-4,5 elimination at the third galacturonic unit from the reducing end of polygalacturonic acid by producing (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1-->4)-(alpha-D-galactopyranosyluronic acid)-(1-->4)-alpha-D-galactopyranuronic acid (3) with a 60% yield. The optimum activity of the enzyme was detected at pH 9.5 and T> or=95 degrees C. The highly thermostable enzyme constitutes a useful catalyst for a simplified synthesis of 4,5-unsaturated trigalacturonic acid 3, a trisaccharide which is extremely difficult to obtain via chemical synthesis.

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Source
http://dx.doi.org/10.1016/s0008-6215(02)00175-1DOI Listing

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