Direct gas chromatographic (GC) analysis of thermally labile N-carbamates is studied by using fast GC with cold on-column injection. With the greatly reduced injection temperature, short column lengths, high flow rate, and fast temperature-programming rate, the exposure of carbamates to high temperature is reduced and degradation can be avoided. Nine N-carbamates in EPA Method 531 are eluted without thermal decomposition. The relative standard deviation percentage for peak areas average 1.9% for all of the carbamates analyzed. A conventional GC instrument is employed to simplify the experiments. GC-mass spectrometry is used to monitor the decomposition peaks.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/chromsci/40.7.321 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Injection artifacts in odorant analysis by gas chromatography.
J Chromatogr A
January 2025
Technical University of Munich, TUM School of Natural Sciences, Department of Chemistry, Lichtenbergstraße 4, 85748 Garching, Germany; Leibniz Institute for Food Systems Biology at the Technical University of Munich (Leibniz-LSB@TUM), Lise-Meitner-Straße 34, 85354 Freising, Germany. Electronic address:
Odor-active compounds are major quality parameters in food and other consumer products. In the analysis of odorants, gas chromatography (GC) plays a dominant role and is particularly indispensable for odorant screening by GC-olfactometry (GC-O). Whereas artifact formation during workup before GC analysis has been widely discussed, artifact formation during GC injection has not been adequately addressed so far.
View Article and Find Full Text PDFImplementations of temperature gradients in temperature-responsive liquid chromatography.
J Chromatogr A
September 2021
Separation Science Group, Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281-S4bis, Ghent B-9000, Belgium. Electronic address:
Temperature Responsive Liquid Chromatography (TRLC) offers an alternative and environmentally friendly way to perform reversed-phase like separations. Its use of temperature responsive polymers to control retention based on column temperature, instead of the fraction of organic modifier in the mobile phase mobile, eliminates the need for solvent composition gradients and allows, for example, for purely aqueous separations. In principle this temperature induced retention should allow for gradient elutions to be performed using downward temperature gradients to control retention and refocus the analyte peaks.
View Article and Find Full Text PDFConcentration Determination of >200 Proteins in Dried Blood Spots for Biomarker Discovery and Validation.
Mol Cell Proteomics
March 2020
University of Victoria - Genome BC Proteomics Centre, University of Victoria, Victoria, British Columbia V8Z 7X8, Canada; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8P 5C2, Canada; Segal Cancer Proteomics Centre, Lady Davis Institute, Jewish General Hospital, McGill University, Montreal, Quebec H3T 1E2, Canada; Gerald Bronfman Department of Oncology, Jewish General Hospital, McGill University, Montreal, Quebec H3T 1E2, Canada; Department of Data Intensive Science and Engineering, Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Nobel St., Moscow143026, Russia. Electronic address:
The use of protein biomarkers as surrogates for clinical endpoints requires extensive multilevel validation including development of robust and sensitive assays for precise measurement of protein concentration. Multiple reaction monitoring (MRM) is a well-established mass-spectrometric method that can be used for reproducible protein-concentration measurements in biological specimens collected via microsampling. The dried blood spot (DBS) microsampling technique can be performed non-invasively without the expertise of a phlebotomist, and can enhance analyte stability which facilitate the application of this technique in retrospective studies while providing lower storage and shipping costs, because cold-chain logistics can be eliminated.
View Article and Find Full Text PDFFischer-Tropsch (FT) wax is an important material produced from FT synthesis reactions. In this study, an improved separation and identification method for FT wax by high temperature gas chromatography (HTGC) coupled with cold-on-column (without pretreatment) was developed. In our improved separation procedure, the carrier gas was changed to helium and a long chromatographic column was adapted for use at high temperature.
View Article and Find Full Text PDFValidation of a HILIC/ESI-MS/MS method for the wood burning marker levoglucosan and its isomers in airborne particulate matter.
Chemosphere
November 2018
Department of Environmental Science and Analytical Chemistry, Stockholm University, SE-106 91, Stockholm, Sweden. Electronic address:
In the present study, a methodology involving hydrophilic interaction liquid chromatography (HILIC) and electrospray (ESI) tandem mass spectrometry (MS/MS) was developed for measurement of anhydrous monosaccharides as markers for wood burning in atmospheric aerosols, PM. No extensive sample preparation, other than ultrasound-assisted solvent extraction and evaporation, was applied. A pentahydroxysilica column enabled separation of levoglucosan from mannosan and galactosan within 5 min and the quantitative performance was validated using the standard reference materials (SRM) 1649a and 1649b.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!